en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Dual-specificity phosphatase 1 (DUSP1) plays a critical role in cellular stress responses, but its function in hepatitis B virus (HBV)-associated hepatocellular carcinoma (LCC) and radiation therapy remains unclear. This study investigates the effects of DUSP1 on HBV replication, tumor progression, and radiation response in LIC. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Materials and Methods:&nbsp;</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Fifteen HBV-LIC patients and 10 radiation-treated liver tumor patients (20&ndash;50 Gy) were included. HepG2.2.15 cells were used to establish DUSP1-overexpressing and control groups. DUSP1 expression was assessed via Western blot and quantitative polymerase chain reaction (qPCR). HBV replication markers (HBsAg, HBeAg) were measured by enzyme-linked immunosorbent assay (ELISA). Cell proliferation cell counting Kit-8 (CCK-8), migration/invasion (Transwell), and miR-21 expression were evaluated. Statistical analysis was performed using SPSS 22.0. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Results: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">DUSP1 was significantly downregulated in LIC tissues (P&nbsp;< 0.001) and further suppressed in irradiated patients (P&nbsp;= 0.002). DUSP1 overexpression reduced HBV replication (HBsAg:&nbsp;P&nbsp;= 0.008; HBeAg:&nbsp;P&nbsp;= 0.008) and enhanced radiosensitivity. Overexpression inhibited proliferation, migration, and invasion (P&nbsp;< 0.001) and attenuated radiation-induced miR-21 upregulation (P&nbsp;= 0.004). </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Conclusion:&nbsp;</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">DUSP1 suppresses HBV replication and tumor progression while enhancing radiation response in LIC, suggesting its potential as a therapeutic target for HBV-associated hepatocellular carcinoma.</span></span></span></span></span></span></span></span></span></span></div> pecificity phosphatases, hepatitis B virus, hepatocellular carcinoma, radiotherapy, microRNA-21, signal transduction. 109 114 http://ijrr.com/browse.php?a_code=A-10-1-1463&slc_lang=en&sid=1 X. Lai 7900319475328460032858 7900319475328460032858 No Department of Liver Disease, Ningbo No.2 Hospital, Ningbo, Zhejiang Province, China G. Gao 7900319475328460032859 7900319475328460032859 No Department of Clinical Laboratory, Ningbo No.2 Hospital, Ningbo, Zhejiang Province, China L. Lin 15988699773@163.com 7900319475328460032860 7900319475328460032860 Yes Department of Liver Disease, Ningbo No.2 Hospital, Ningbo, Zhejiang Province, China