en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Explore the effects of different concentrations of nicotine on the microstructure of rat alveolar bone and the expression of osteogenic related proteins, in order to clarify its role in alveolar bone osteogenic metabolism. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Materials and Methods:</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB"> In this study, 28 8-week-old male Sprague Dawley (SD) rats were randomly divided into four groups (control group, low-dose group, medium dose group, high-dose group) and subjected to continuous intraperitoneal injection intervention for 180 days. Subsequently, the microstructure characteristics of alveolar bone were analyzed using micro computed tomography (Micro-CT), and the expression of osteogenic related proteins was accurately determined by combining immunohistochemistry and qRT PCR techniques. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Results: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Nicotine dose dependently reduces the volume of rat alveolar bone and damages its microstructure; Nicotine dose dependently reduces the expression of type I collagen (COL-I), alkaline phosphatase (ALP), osteocalcin (OCN), and bone sialoprotein (BSP), while increasing the expression of osteopontin (OPN). </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Conclusion: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Nicotine interferes with the osteogenic process of rat alveolar bone by affecting the expression of osteogenic related proteins.&nbsp;</span></span></span></span></span></span></span></span></span></span></div> Nicotine, alveolar bone, osteogenesis-related proteins, microstructure. 405 410 http://ijrr.com/browse.php?a_code=A-10-1-1509&slc_lang=en&sid=1 X. Li dlxm19921fs@163.com 7900319475328460033507 7900319475328460033507 No Department of Caries and Endodontics, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China J. Mi 7900319475328460033508 7900319475328460033508 No Department of Caries and Endodontics, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China Y. Zhang 7900319475328460033509 7900319475328460033509 No Department of Caries and Endodontics, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China