International Journal of Radiation Research

en Reduction of cat allergen levels based on anti-Fel d1 andanti-Fel d4 IgY after radiotherapy Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Fel d 1 and Fel d 4 are the major feline allergens responsible for allergic responses in sensitized individuals. Immunoglobulin Y (IgY) antibodies can neutralize allergens, while low-dose radiotherapy may transiently alter glandular secretion. To evaluate the combined effect of anti-Fel d 1 and anti-Fel d 4 IgY and controlled low-dose radiotherapy on reducing allergen levels in cats. Materials and Methods: Cats received localized low-dose radiotherapy (2 Gy delivered in two fractions) followed by oral supplementation with purified and lyophilized anti-Fel d 1 and anti-Fel d 4 IgY. Allergen concentrations in saliva and hair were measured weekly over six weeks using ELISA. IgY purity, titer stability, and neutralizing activity were assessed in-vitro. Results: IgY purity ranged from 80–90% and titers remained stable after lyophilization. Radiotherapy alone produced a transient 10–15% reduction in salivary allergen secretion. With IgY administration, salivary Fel d 1 decreased from 4.50 μg/mL to 0.28 μg/mL (−94%) and hair Fel d 1 from 1.30 μg/mL to 0.15 μg/mL (−88%) by week six. Salivary Fel d 4 decreased from 12.0 μg/mL to 1.70 μg/mL (−86%). No radiation-related adverse effects were observed. Conclusion: Low-dose radiotherapy produced a short-term decrease in allergen secretion, while IgY supplementation resulted in a sustained and substantial reduction of Fel d 1 and Fel d 4 levels. This combined approach may represent a practical strategy for reducing environmental cat allergen exposure.</div> Fel d 1, Fel d 4, immunoglobulins, radiotherapy, cat allergens, hypersensitivity, enzyme-linked immunosorbent assay. 487 493 http://ijrr.com/browse.php?a_code=A-10-1-1521&slc_lang=en&sid=1 W. Chai 7900319475328460033601 7900319475328460033601 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China S. Han 7900319475328460033602 7900319475328460033602 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China M. Deng 7900319475328460033603 7900319475328460033603 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China J. Ma 7900319475328460033604 7900319475328460033604 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China Y. Li 7900319475328460033605 7900319475328460033605 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China Q. Shi 7900319475328460033606 7900319475328460033606 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China W. Zhang 7900319475328460033607 7900319475328460033607 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China Y. Xu 7900319475328460033608 7900319475328460033608 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China M. Dong Mxdong64@qdu.edu.cn 7900319475328460033609 7900319475328460033609 Yes School of Pharmacy, Qingdao University, Haoyuan Medical Education Comprehensive Building, Fushan Campus, Qingdao City, China Z. Dai daizhihong@bio-ss.net 7900319475328460033610 7900319475328460033610 No Department of Research / Technology, Ningbo Sansheng Biotechnology Co., Ltd., Wangchun Industrial Zone, Haishu District, Ningbo City, Zhejiang Province, China