en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">The ISG15 and CD3 play roles in immune regulation and cancer biology, but their prognostic and therapeutic significance in gastric cancer (GC) remains unclear. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Materials and Methods: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">ISG15 expression was evaluated using TCGA and GTEx datasets across various cancers by enrichment and immune infiltration analyses. Immunohistochemistry was performed to assess ISG15 and CD3 expression and their associations with clinicopathological features, prognosis, and correlation with each other on 103 GC specimens. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Results: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">ISG15 was overexpressed in multiple cancers, including GC, and correlated with immune cell infiltration. In the clinical cohort, ISG15 was highly expressed in 68.93% of cases, while CD3 was low in 89.32%. High ISG15 expression correlated with prolonged APTT, whereas high CD3 expression was associated with HER-2 mutation, elevated CA-125 and PCT, and reduced Hb and TC, all indicators of poor prognosis. Both ISG15 and CD3 expression correlated with shorter progression-free survival. A significant positive correlation between ISG15 and CD3 expression was observed. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Conclusion: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Elevated ISG15 and CD3 predict adverse clinicopathological features and prognosis in GC. Given their roles in immune regulation and DNA damage responses, ISG15 and CD3 may also serve as biomarkers of radiosensitivity, radiotherapy and combined radio-immunotherapy strategies.</span></span></span></span></span></span></span></span></span></span></div> Gastric cancer, ISG15 protein, CD3 antigens, radiosensitivity, tumour microenvironment, prognosis. 545 552 http://ijrr.com/browse.php?a_code=A-10-1-1537&slc_lang=en&sid=1 Y. Huang ArthEynolds6017@hotmail.com 7900319475328460033770 7900319475328460033770 Yes Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China H.H. Liu 7900319475328460033771 7900319475328460033771 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China Y. Liang 7900319475328460033772 7900319475328460033772 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China X.Z. Xu 7900319475328460033773 7900319475328460033773 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China Z.H. Che 7900319475328460033774 7900319475328460033774 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China Y.Z. Lin 7900319475328460033775 7900319475328460033775 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China X.Q. Yu 7900319475328460033776 7900319475328460033776 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China X. Li 7900319475328460033777 7900319475328460033777 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China J.Y. Liang 7900319475328460033778 7900319475328460033778 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China T. Feng 7900319475328460033779 7900319475328460033779 No Department of Nephrology, The People’s Hospital of Wuzhou, Wuzhou, 543001, Guangxi, China