International Journal of Radiation Research

en Effects of ginsenoside Rg3 on chemoradiosensitivity to paclitaxel in breast cancer cells and its correlation with autophagy-related genes Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Resistance to paclitaxel (PTX) remains is a challenge in treatment of breast cancer, which is often mediated by multidrug resistance proteins and dysregulated autophagy. Ginsenoside Rg3 has been reported to enhance chemosensitivity in several malignancies, but its role in PTX resistance and underlying mechanisms in breast cancer remain unclear. Materials and Methods: In this study Human breast cancer MCF-7 cells treated with PTX, Rg3, or both. Cell viability was assessed by MTT assay, apoptosis by Annexin V/PI flow cytometry, and drug efflux by rhodamine-123 retention. Autophagy was evaluated using transmission electron microscopy, monodansylcadaverine and acridine orange staining, and Western blot analysis of autophagy-related proteins. Expression of MDR1/P-glycoprotein and Wnt/β-catenin signaling components was also analyzed. Results: Rg3 significantly enhanced PTX-induced growth inhibition and apoptosis in MCF-7 cells compared with PTX alone. Combination treatment increased intracellular rhodamine-123 accumulation, indicating reduced drug efflux. Autophagy activation was evidenced by increased LC3-II/LC3-I ratio and Beclin-1 expression, along with decreased p62 levels. Additionally, Rg3 combined with PTX downregulated MDR1/P-glycoprotein and suppressed key proteins of the Wnt/β-catenin signaling pathway. Correlation analysis revealed that autophagy markers were positively associated with apoptosis and negatively correlated with MDR1 expression. Conclusion: Ginsenoside Rg3 increases breast cancer cells chemosensitivity to paclitaxel, potentially through modulation of autophagy, inhibiting Wnt/β-catenin signaling, and suppressing multidrug resistance mechanisms. The findings of this study showed that Rg3 may represent a potential adjuvant for chemotherapy, while its role in modulating radiosensitivity needs further investigation in dedicated radiotherapy models.</div> Ginsenoside Rg3, paclitaxel, breast cancer, autophagy, drug resistance Wnt/β-catenin, radiosensitization, radiotherapy-related resistance. 713 721 http://ijrr.com/browse.php?a_code=A-10-1-1568&slc_lang=en&sid=1 S. Chen 7900319475328460034298 7900319475328460034298 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China Y. Liu 7900319475328460034299 7900319475328460034299 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China H. Bao 7900319475328460034300 7900319475328460034300 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China E. Zhao 7900319475328460034301 7900319475328460034301 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China X. Qiao 7900319475328460034302 7900319475328460034302 No School of Pharmacy, Qiqihar Medical College, Qiqihar, Heilongjiang Province, China M. Gu 7900319475328460034303 7900319475328460034303 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China J. Zhou 7900319475328460034304 7900319475328460034304 No Department of Thoracic Surgery and Department of Tumor Surgery II, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China J. Zhang zhangjingyan1982@126.com 7900319475328460034305 7900319475328460034305 Yes