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:: Volume 20, Issue 1 (1-2022) ::
Int J Radiat Res 2022, 20(1): 15-20 Back to browse issues page
LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radio-sensitivity
X.N. Yu, M. Wang
Department of Oncology, Weihai Central Hospital, Weihai, 264400, China , wangming99801@163.com
Abstract:   (1046 Views)
Background: To investigate whether LncRNA LINC01204 affects lung cancer cell apoptosis, migration, invasion and radiosensitivity by regulating miR-214. Materials and Methods: Normal lung cells HBE and lung cancer cells A549 were cultured in-vitro, and pcDNA3.1, pcDNA3.1-LINC01204, pcDNA3.1-LINC01204 and miR-NC, pcDNA3.1-LINC01204 and miR-214 mimics were transfected into A549 cells, respectively. qRT-PCR, flow cytometry, scratch test, and Transwell chamber test, clone formation test, dual luciferase, and Western blot methods were used in this study. Results: Compared with HBE cells, A549 cells had significantly reduced LINC01204 expression level (P<0.05), and significantly increased miR-214 expression level (P<0.05); transfection of pcDNA3.1-LINC01204 could significantly increase apoptosis rate and Cleaved-caspase3, E-cadherin protein levels (P<0.05), reduce migration healing rate, N-cadherin protein levels and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 1.950, reduce the number of invasive cells (P<0.05). Dual luciferase report experiments confirmed that LINC01204 can bind to miR-214 in a targeted way; co-transfection of pcDNA3.1-LINC01204 and miR-214 mimics can significantly reduce the apoptosis rate and Cleaved-caspase3 and E-cadherin protein levels (P<0.05), improve the migration healing rate, N-cadherin protein level and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 0.728, increase the number of invasive cells (P<0.05). Conclusion: LINC01204 overexpression can negatively regulate miR-214 expression to promote lung cancer cell apoptosis and inhibit migration and invasion, thereby enhancing radiosensitivity.
 
Keywords: LncRNA LINC01204, miR-214, lung cancer, apoptosis, migration, invasion, radiosensitivity.
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Type of Study: Original Research | Subject: Radiobiology
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Yu X, Wang M. LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radio-sensitivity. Int J Radiat Res. 2022; 20 (1) :15-20
URL: http://ijrr.com/article-1-4060-en.html


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Volume 20, Issue 1 (1-2022) Back to browse issues page
International Journal of Radiation Research
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