[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 12, Issue 3 (7-2014) ::
Int J Radiat Res 2014, 12(3): 211-215 Back to browse issues page
Cytokinesis blocked micronucleus (CB-MN) assay for biodosimetry of high dose accidental exposure
K. Shirsath , N. Bhat , K. Anjaria , U. Desai , S. Balakrishnan
Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India , bsdevi@barc.gov.bsdevi@barc.gov.bsdevi@barc.gov.in
Abstract:   (7830 Views)

Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytochalasin-B was added to the cultures at 44 h. Slides were analyzed for frequency of Mononucleated cells (MNC), ratio of Tri/Quadra nucleated cells, micronuclei ( MN ) yield in MN positive cells, mitotic bridges and apoptosis. Results: At 5 Gy 67% Mononucleated cells, 0.22% apoptotic cells and 1.76% mitotic bridges were observed. Micronuclei yield in MN positive binucleated cells was found to be 1.43±0.04. At 8Gy 96.63% Mononucleated cells, 22.64% apoptotic cells and 3.8% mitotic bridges were observed. Micronuclei yield in MN positive binucleated cells was found to be 2.18±0.18. At 15 Gy, 99.6% of the cells were Mononucleated and 0.38% was binucleated and MN yield was 2.9 ±0. The total number of MN in MN positive cells increases with increasing radiation doses. Conclusion: All the three indicators number of MN in all binucleated cells (BNC) , number of MN /MN positive binucleated cells and the nuclear division index (NDI) can serve as good biomarkers for biodosimetry at high doses. Although the frequency of mitotic bridges and apoptotic bodies increases with dose, there is no consistent difference between various doses. Thus the CBMN + NDI assay can be a biodosimetry method at high doses.

Keywords: CB, MN assay, biodosimetry, high dose exposures
Full-Text [PDF 458 kb]   (1863 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shirsath K, Bhat N, Anjaria K, Desai U, Balakrishnan S. Cytokinesis blocked micronucleus (CB-MN) assay for biodosimetry of high dose accidental exposure. Int J Radiat Res 2014; 12 (3) :211-215
URL: http://ijrr.com/article-1-1277-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 3 (7-2014) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.06 seconds with 50 queries by YEKTAWEB 4645