[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 12, Issue 3 (7-2014) ::
Int J Radiat Res 2014, 12(3): 223-227 Back to browse issues page
Establishment of a rat model of radiation-induced lung injury
S Qibin , H. Weiguo , C. Yuxin , Xubin , C. Hao
Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China , 347952582@qq.com
Abstract:   (5729 Views)

Background: Radiation-induced lung injury is a refractory side effect in lung cancer radiotherapy, the mechanism still remains unclear, hence an appropriate animal mode may become useful to investigate it. Materials and Methods: 50 female Wistar rats were randomly divided into 5 groups, average 10 rats/cage: A. control group B. 3Gy×10f C. 6Gy×5f D. 12.5Gy×1f E.15.3Gy×1f. By different doses of radiation to the right lung of rats under American Varian linear accelerator, all the rats were executed at 30days after irradiation, lung tissues were collected to observe the pathological changes by HE staining , and additional lung tissues were extracted mRNA to measure gene expression of TNF-a and IL-18 by qRT-PCR. Results: In contrast to the control group, congestion, gore, edema, broadening alveolar interval and the bulla can be observed after irradiation. 3Gy×10f radiation led to most rat death because of frequent anaesthesia. 12.5Gy×1f radiation induced mild lung injury. 6Gy×5f and 15.3Gy×1f radiation induced remarkable lung injury. However, 15.3Gy×1f was seldom adopted in clinical settings. The measurement of cytokine variation by qRT-PCR also indicated that 6Gy×5f radiation stimulated the most obvious elevation of IL-18, while 15.3Gy×1f radiation elevated TNF-a most. Conclusion: A rat model of radiation-induced lung injury was appropriately established, 6Gy×5f radiation was the most suitable radiation method for establishing this rat model.

Keywords: Rat, radiation, lung injury, model
Full-Text [PDF 801 kb]   (2219 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Qibin S, Weiguo H, Yuxin C, Xubin, Hao C. Establishment of a rat model of radiation-induced lung injury. Int J Radiat Res 2014; 12 (3) :223-227
URL: http://ijrr.com/article-1-1279-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 3 (7-2014) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 50 queries by YEKTAWEB 4645