Trabecular bone changes induced by fast neutrons versus gamma rays in mice
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Y. Yang , H.J. Lee , H. Jang , J.H. Lee , C. Moon , J.C. Kim , U. Jung , S.K. Jo , C.M. Kang , S.H. Kim  |
College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea , shokim@chonnam.ac.kr |
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Abstract: (7933 Views) |
Background: The trabecular bone changes in the tibia of C3H/HeN mice were measured 12 weeks after whole body irradiation with various doses of fast neutrons (0-2.4 Gy) or 137Cs-generated gamma-rays (0-6 Gy). Materials and Methods: Serum calcium, phosphorus, estradiol concentration and alkaline phosphatase activity were measured. Tibiae were analyzed using microcomputed tomography. Biomechanical property and osteoclast surface level were measured. Results: There was a significant relationship between the loss of bone architecture and the radiation dose, and the best-fitting dose-response curves were linear-quadratic. Mean relative biological effectiveness (RBE) values (Ref. gamma) of 2.05 and 2.33 were estimated for fast neutron irradiation in trabecular bone volume fraction and bone mineral density, respectively. There was a substantial reduction in osteoclast surface level in tartrate-resistant acid phosphatase-stained histological sections of tibial metaphyses in irradiated mice with high dose of neutrons. Conclusion: There was a significant relationship between the loss of bone architecture and the radiation dose. The difference of osteoclastic bone resorption may represent a contributor to the low RBE in high dose of irradiation level relative to that of low dose level. |
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Keywords: Neutrons, trabecular bone, osteoclast, relative biological effectiveness |
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Full-Text [PDF 1237 kb]
(1661 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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