:: Volume 15, Issue 2 (4-2017) ::
Int J Radiat Res 2017, 15(2): 213-218 Back to browse issues page
Human amniotic membrane effect on colorectal anastomosis in dogs undergoing radiotherapy with/without diverting stoma: An experimental preliminary study
S.V. Hosseini , M. Dehghani , H. Khazraei Dr. , M.Y. Karami , S. Karbasi , S.H. Banihashmi
Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , hajarkhazraei@gmail.com
Abstract:   (1772 Views)

Background: : Radiotherapy is an important factor which results in increase of anastomosis leakage. Diverting loop ileostomy has been usually performed after colorectal anastomosis with history of neo-adjuvant radiotherapy to decrease the chance of leakage. Considering this effect, we assessed the feasibility and outcome of human amniotic membrane in rectal anastomosis in dogs previously treated by high-dose radiotherapy. Materials and Methods: Twelve cross-breed male dogs with the age of 6-8 months and weighting 10-15 kg were randomly divided into four groups. Groups 1 and 2 received radiotherapy and 4 weeks later, single layer end to end anastomosis was performed for all the dogs; also, diverting loop ileostomy was placed in groups 1 and 3. Four weeks later, the anastomosis site was resected and sent for pathologic wound healing scoring. Data were analyzed by SPSS 16 software using Mann- Whitney test. Results: Two dogs (of group 1 and 2) died of peritonitis due to anastomosis leakage during the 2nd post operation week. There was no statistically significant difference in wound healing between the case and control groups (P-Value: 0.01). Conclusion: HAM had a protective role in colorectal anastomosis after neo-adjuvant radiation in cases without loop diverting ileostomy. It can be concluded that HAM placement is a feasible technique instead of diverting loop ileostomy in cases with neo-adjuvant radiotherapy and also in the benign colorectal diseases with high risk anastomoses.

Keywords: Human amniotic membrane, radiation therapy, anastomosis leakage, ileostomy.
Full-Text [PDF 683 kb]   (288 Downloads)    
Type of Study: Reviews and Perspectives | Subject: Radiation Biology

XML     Print

Volume 15, Issue 2 (4-2017) Back to browse issues page