:: Volume 16, Issue 1 (1-2018) ::
Int J Radiat Res 2018, 16(1): 123-127 Back to browse issues page
Determination of hemolysis, osmotic fragility and fluorescence anisotropy on irradiated red blood cells as a function of kV of medical diagnostic X-rays
M. Tungjai Dr. , N. Phathakanon , P. Ketnuam , J. Tinlapat , S. Kothan
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroroj Rd., Sripoom, Chiang Mai, 50200, Thailand , mtungjai@gmail.com
Abstract:   (1181 Views)
Background: People occasionally undergo medical diagnostic X-ray examinations and expose their red blood cells to radiation. Radiation that is generated from medical diagnostic X-ray machines is widely used in medical diagnoses. One of the important parameters is kilo-voltage (kV) that is applied across the X-ray tube in medical diagnostic X-ray machines. Kilo-voltage influences the radiation dosage. The aim of this study is to determine the hemolysis, osmotic fragility, and fluorescence anisotropy value on irradiated red blood cells as a function of kV during medical diagnostic X-ray examinations.  Materials and Methods: The kV, kilo-voltage that is applied across an  X-ray tube, of a medical diagnostic X-ray machine was operated at 50, 70 and 100 kV. We determined the hemolysis, osmotic fragility, and fluorescence anisotropy value in red blood cells at 0.5 and 4 hours post-irradiation. In order to determine hemolysis and osmotic fragility, the release of hemoglobin was measured by spectrophotometry technique. 1,6-diphenyl-1,3,5-hexatriene (DPH) was used as a molecular probe for determining fluorescence anisotropy value by fluorescence anisotropy technique. Non-irradiated red blood cells served as the control. Results: For the 50, 70, and 100 kV of medical diagnostic X-rays, the hemolysis, osmotic fragility, and fluorescence anisotropy values of irradiated red blood cells at 0.5 and 4 hours post-irradiation did not significantly change when compared to the control.  Conclusion: Our results suggested that 50, 70, and 100 kV of medical diagnostic X-ray did not influence hemolysis, osmotic fragility, and fluorescence anisotropy values of irradiated red blood cells.
 
Keywords: Medical diagnostic X-ray, red blood cell, hemolysis, osmotic fragility, fluorescence anisotropy.
Full-Text [PDF 1190 kb]   (574 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology


XML     Print



Volume 16, Issue 1 (1-2018) Back to browse issues page