Neurofibroma of the prostate is an extremely rare benign tumor, particularly when it is not associated with neurofibromatosis-I (NF-1). This study aimed to report the case of a 44-year-old man who presented with dysuria for about 4 years. Magnetic resonance imaging (MRI) showed a mass in the prostate with “target sign” on T2-weighted imaging. The mass was surgically excised, and histopathological findings revealed a neurofibroma. No clinical or laboratorial manifestations of NF-1 were identified. Also, no local recurrence occurred after the 3-month follow-up. This was the first case of prostate solitary neurofibroma without NF-1, and the “target sign” on MRI could supply some significant implications in the diagnosis.