Background: The effect of ionizing radiation of 8-HYDROXY- 2’- DEOXYGUANOSINE (8-OHdG) level in radiographers’ urine as the oxidative damage biomarker was investigated in this study and this biomarker was compared with the collective effective doses. Materials and Methods: The samples were selected into two categories in this cross-sectional study, 35 of whom were from different radiography groups (including nuclear medicine, radiology, radiotherapy, CT scan), and 35 subjects were from the staff who had no exposure to radiation. The results of the film badge were gathered from the hospitals, and the collective effective dose was obtained according to the respective formula. Then, at the end of the work shift, the urine samples were taken to determine the 8-OHdG concentration. The samples were obtained via the SPE (solid-phase extraction) method. After that, the 8-OHdG concentration was read by the GC/MS analyzer. Finally, the data resulted from the 8-OHdG concentration and the collective effective dose of the radiation were analyzed by the SPSS software. Result: The results showed that the 8-hydroxy-2-deoxy-guanosine concentration in the urine of the radiation workers had significant correlation with the collective effective dose of the radiation the past 6 periods (P=0.009) and also with the collective effective dose of the radiation of the 30 last periods (P=0.009). Conclusion: Observing the radiation protection principles by radiation workers results in decreased radiation and, in turn, reduces the level of oxidative stress, thus, reducing the potential effects of radiation.