:: Volume 21, Issue 1 (1-2023) ::
Int J Radiat Res 2023, 21(1): 73-78 Back to browse issues page
Immunomodulatory effects of ionizing radiation on peripheral blood mononuclear cells
N. Öztürk , A. Karlıtepe , B. Depboylu , M. Kılıç Eren
Aydın Adnan Menderes University, Faculty of Medicine, Department of Medical Biology, Aytepe Campus, Aydin, Turkey , mkilic@adu.edu.tr
Abstract:   (652 Views)
Background: Avoiding exposure to ionizing radiation due to environmental factors is almost inevitable in daily life. Here, we aimed to investigate the possible immunomodulatory effects of ionizing radiation on NK and T cell activation using Peripheral Blood Mononuclear Cells (PBMC). We measured the pro-inflammatory cytokines INFγ, IL-2 and TNFα, as well as Granzyme B. In addition, we determined the expression levels of CD28, NKG2D (CD314) receptors, which play a key role in the activation of T and NK cells, respectively. Materials and Methods: 20 ml peripheral blood samples were taken from healthy volunteer donors and exposed to radiation doses of 0, 1, 3 and 5 Gy. ELISA analysis was used to measure Granzyme B, INFy, TNFα and IL2. Expression of CD28, NKG2D (CD314) receptors was measured by qRT-PCR analysis. Apoptosis and necrosis were measured by AnnexinV/7AAD analysis. Catalase activity was measured using hemolysates from irradiated blood samples. Results: Here we show that IR exposure induces necrotic cell death in PBMCs as the main response. IR exposure significantly induced secretions of Granzyme B, TNFα, IL2, and INFγ in a dose-dependent manner. In addition, mRNA levels of CD28 and NKG2D expressions were increased by 3 Gy IR exposure, but decreased by 5 Gy, while catalase activity increases with 1 Gy IR treatment, 3 and 5 Gy decreases. Conclusions: Our results suggest that not only high doses but even low doses of radiation can modulate the immune response through cytokine secretion and activation of T and NK cell receptors.
Keywords: Ionizing radiation, immune cells, necrotic cell death, CD28, NKG2D.
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Type of Study: Original Research | Subject: Radiation Biology
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