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YCF and YAP gene expressions in yeast cells after irradiation combined with mercury treatment
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S.H. Kim   , J. K. Kim |
| , jkkim@kaeri.re.kr |
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Abstract: (14892 Views) |
| Background: All aerobically growing organisms
suffer from exposure to oxidative stress, caused by
partially reduced forms of molecular oxygen, known
as reactive oxygen species (ROS). These are highly
reactive and capable of damaging cellular constituents
such as DNA, lipids and proteins. Consequently,
cells from many different organisms have evolved
mechanisms to protect their components against
ROS. It is known to have some genes for resistance to
heavy metals and ionizing radiation (IR). Materials
and Methods: Saccharomyces cerevisiae is an ideal
model organism for deducing biological processes in
human cells. In this work, cell viability and gene
expression was investigated in yeasts treated with IR,
HgCl2, and IR combined with HgCl2. Cell viability was
measured by colony forming unit (CFU) method in an
YPD medium. Gene expression was analyzed by the
Real-time PCR. Results: The viability was lower at the
higher dose. At a dose above a certain level, the
viability came down to zero. The combined treatment
decreased the viability, as well. Metal resistance
genes were expressed in the cells treated with HgCl2.
In a similar way, irradiation also triggered the expression
of some radiation resistance genes. YCF and YAP
genes were induced consecutively with the HgCl2
concentration, and also with a higher total dose
under a lower dose rate condition. These two genes
were, however, expressed differently under the 0.2
mM HgCl2 treatment condition. In the cells treated
with 0.1-0.2 mM HgCl2, the viability was higher than
with any other concentrations. Conclusion: The
results demonstrated that the higher dose induced
more expression of oxidative stress resistance genes
related to cell survival mechanism. Combined treatment
of radiation with mercury chloride resulted in
synergistic effects leading to a higher expression of
the genes than treatment of a single stressor alone.
Iran. J. Radiat. Res., 2009 6 (4): 161-166 |
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| Keywords: Metalloid Stress, ionizing radiation, YCF gene, YAP gene, yeast, ROS. |
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Full-Text [PDF 369 kb]
(2540 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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