Modification of 10 cGy neutron or gamma-rays induced chromosomal damages by hyperthermia: an in vitro study
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D. Fatehi , H. Mozdarani |
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Abstract: (10942 Views) |
Background: To evaluate the effects of
hyperthermia (HT) on the frequency of chromosomal
aberrations induced by a low dose of neutron or
γ-rays in human peripheral blood lymphocytes.
Materials and Methods: Blood samples were exposed
to HT (41.5°C for 30 and 60min, 43°C for 15 and
30min), 10 cGy neutron or γ-rays, HT + neutron/γ,
and neutron/γ + HT. After standard cell culture,
harvesting, fixation and staining, the chromosomal
damages were scored in metaphase plates. Results:
HT alone at 41.5°C did not induce chromatid or
chromosome aberrations, however, the frequency of
damages was significantly higher at 43°C (P<0.05).
Furthermore, the chromosomal damages was
significantly different when cells were irradiated with
neutron or γ-rays alone (P<0.01). HT 1 hr post
neutron/γ irradiation significantly induced higher
chromosome damages in comparison to HT 1 hr
before irradiation (P<0.05). The chromosomal
damages were remarkably higher when cells
were irradiated with neutron then heated at 43°C for
30 min. Conclusion: Since increasing frequency of
chromosome damages increases probability of cell
death, application of HT after neutron irradiation
(instead of X– or γ- rays) might be considered as a
procedure for cells killing in radiotherapy. Iran. J.
Radiat. Res., 2009 7 (2): 69-77 |
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Keywords: Hyperthermia, neutron, gamma rays, chromosomal aberration, cancer. |
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Full-Text [PDF 377 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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