Ameliorating effects of genestein: Study on mice liver glutathione and lipid peroxidation after irradiation
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A. Gaur |
, ajay_gaur11@yahoo.com |
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Abstract: (26246 Views) |
Background: Genistein is a soya isoflavone,
which is found naturally in legumes, such as
soybeans and chickpeas. Radiation–induced free
radicals in turn impair the antioxidative defense
mechanism, leading to an increased membrane lipid
peroxidation that results in damage of the membrane
bound enzyme and may lead to damage or death of
cell. Hence, the lipid peroxidation is a good biomarker
of damage occurs due to radiation and the inhibition
of lipid peroxidation is suggestive of radioprotective
action. Glutathione has been shown to protect cells
against oxidative stress by reacting with peroxides
and hydroperoxides and determines the inherent
radiosensitivity of cells. Materials and Methods: For
experimentation, healthy Swiss Albino male mice of 6
-8 weeks old were selected from inbred colony.
Genistein was dissolved in dimethyl sulfoxide and
then prepared different concentration solutions so
that the volume administered intraperitoneally was
0.5 ml. Lipid peroxidation was estimated by the
method of Ohkawa and GSH was estimated by the
method of Moron. Results: The intraperitoneal
administration of optimum dose (200 mg/kg body
weight) of Genistein before 24 hrs and 15 minutes of
irradiation (8 Gy at a dose rate of 1.02 Gy/min)
reverted the increase in lipid peroxidation (by 18.01%
± 3.05) and decrease of Glutathione (by 62.05% ±
21.58) caused by irradiation in liver of Swiss albino
mice. Statistically analyzed survival data produced a
dose reduction factor (DRF) = 1.24. Conclusion: The
results indicate that Genistein against radiation effect
may pave way to the formulation of medicine in
radiotherapy for normal tissue and possible against
radiomimetic drug induced toxicity. Iran. J. Radiat.
Res., 2009 7 (4): 187-199 |
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Keywords: Genistein, tyrosine kinase inhibitor, radiation, liver, oxidative stress. |
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Full-Text [PDF 313 kb]
(3883 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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