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Evaluation of combination effects of 2- methoxyestradiol and methoxyamine on IUdRinduced radiosensitization in glioma spheroids
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A. Neshasteh-Riz   , S. Babaloui , S. Khoei |
| , neshastehriz@yahoo.com |
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Abstract: (14813 Views) |
| Background: Glioblastoma is the most common
and most malignant cancer of central nervous
system. Targeted radiotherapy is an effective method
toward its treatment. Iododeoxyuridine (IUdR) is a
halogenated thymidine analogue known to be
effective as a radiosensitizer in human cancer
therapy. In this study we have evaluated the combination
effects of 2-Methoxyestradiol, an inhibitor of
hypoxia inducible factor 1α (HIF-1α) and
Methoxyaminem, an inhibitor of base excision repair
(BER) pathway on radiosensitization of IUdR in
glioblastoma spheroid culture. Materials and
Methods: The cytotoxic damages of DNA in U87MG
cell line were compared using colony formation assay.
Experiments were performed in large spheroids with
a diameter of approximately 350μm. Results:
Evaluation of the effects of IUdR with 2ME2 and MX
pretreatment on spheroid cultured cell followed by
ionizing irradiation showed more enhancemented
(p≤0.001) IUdR induced-radiosensitization. These
results introduced a key role for 2ME2 in IUdR related
studies. Conclusion: Pretreatment of tumor cells with
IUdR, MX and 2ME2 before irradiation enhances
tumor radiosensitization and may improve therapeutic
index for IUdR and 2ME2. Iran. J. Radiat. Res.,
2010 7 (4): 211-216 |
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| Keywords: Hypoxia-inducible factor-1α, IUdR, radiosensitization, spheroid, methoxyamine, 2-methoxyestradiol. |
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Full-Text [PDF 469 kb]
(2362 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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