Modulation of radiation induced changes in nucleic acid content of liver of Swiss albino mouse by Tinospora cordifolia (Miers)
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S. Arya , J. Sharma |
, sharma_jaimala@yahoo.co.in |
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Abstract: (10415 Views) |
Background: Radiotherapy is the main modality
of cancer treatment. There are many chemical radioprotectors
which unfortunately have lethal or toxic
effect. Therefore the search is on to find out natural
plant based radioprotectors. A well known medicinal
plant,which is more acceptable to the body,
Tinospora cordifolia, was tested in animal tissues
against gamma radiations. Radioprotective effects of
Tinospora cordifolia (Miers) extract against radiation
induced biochemical changes in liver of Swiss albino
mouse were investigated. Materials and Methods: For
experimental study, healthy swiss albino mice were
selected from an inbred colony and divided into six
groups and exposed to 6Gy and 8Gygamma radiation
(control) or 6Gy and 8Gy gamma radiation with 5mg/
kg body weight of TC extract (experimental), sham
irradiated (Normal) and plant extract only. Mice were
sacrificed at various post irradiation intervals and
liver was removed for quantitative estimation of DNA
and RNA. Results: On the first day post irradiation in
control group (6Gy and 8Gy both). DNA content
decreased significantly as compared to the sham
irradiated controls. Then there was continuous
increase uptill 28th day but it remained below the
normal. Decrease in DNA content of liver in the
experimental group (6Gy) was observed on 1st day
but the values were higher than that of the controls.
RNA content increased in the control animals treated
with 6Gy and 8Gy which was maximum at day 3,
followed by a decrease at the subsequent intervals.
Increase in the amount of RNA was recorded in the
experimental animals also. Then came down to the
normal on 10th day in the experimental groups
(P<0.01). Conclusion: These results indicate that
TC is able to protect nucleic acids the liver of Swiss
albino mouse against gamma radiation. Iran. J.
Radiat. Res., 2010 8 (3): 179-185 |
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Keywords: Radioprotection, tinospora cordifolia, liver, DNA, RNA. |
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Full-Text [PDF 270 kb]
(2739 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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