[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 8, Issue 4 (3-2011) ::
Int J Radiat Res 2011, 8(4): 243-248 Back to browse issues page
Synthesis and biodistribution study of a chlorotoxin derivative peptide labeled with 131- iodine for tumor therapy
S.P. Shirmardi , M. Shamsaei Dr. , M. Gandomkar , M. Ghannadi Maragheh
, pysham@aut.ac.ir
Abstract:   (10145 Views)
Background: Chlorotoxin is a 36-amino acid peptide found in the venom of the Leiurus quinquestriatus which blocks small-conductance chloride channels. Chlorotoxin binds preferentially to glioma cells that allow development of new methods for the treatment and diagnosis of several types of cancer. Thus chlorotoxin derivative was labeled with 131I for further investigation. Materials and Methods: A chlorotoxin derivative was synthesized on a solid phase using a standard Fmoc strategy. Labeling with iodine-131 was performed through chloramine-T method and radiochemical analysis involved sephadex G-25 and HPLC methods. The stability of radiopeptide was checked in the presence of PBS and human serum at 37 °C up to 24 h. The biodistribution was studied in mice. Results: The chemical purity of synthesized peptide as assessed by analytical RP-HPLC was 95%. Labeling of peptide resulted in a radiochemical yield of 80% with radiochemical purity of > 95% with specific activity of 0.740 GBq/μmol. Result of in vitro studies demonstrated acceptable stability of compound in human serum and PBS solution. Biodistribution data showed moderate blood clearance, with concentration of radioactivity in the kidneys, liver, intestine and stomach. Conclusion: Results indicates that the labeled Chlorotoxin derivative might be useful in determining tumor extent and also, tumor therapy of gliomas or possibily other cancers. Iran. J. Radiat. Res., 2011 8(4): 243-248
Keywords: Cancer, peptide, 131I, labeling, chlorotoxin.
Full-Text [PDF 342 kb]   (2385 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shirmardi S, Shamsaei M, Gandomkar M, Ghannadi Maragheh M. Synthesis and biodistribution study of a chlorotoxin derivative peptide labeled with 131- iodine for tumor therapy. Int J Radiat Res. 2011; 8 (4) :243-248
URL: http://ijrr.com/article-1-687-en.html


Volume 8, Issue 4 (3-2011) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3638