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Effects of gamma irradiation on antioxidant activity of Ergosan
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M. Heidarieh   , A. Borzouei , S. Rajabifar , F. Ziaie , Sh. Shafiei |
| , mheidarieh@nrcam.org |
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Abstract: (11451 Views) |
| Background: The approaches chosen for control
of Outbreaks of infectious diseases in Aquatic
farming industry include improvement of environmental
conditions, stocking of specific pathogen free
(SPF) brood stockings, and application of vaccines
and immunostimulants. Despite numerous studies on
the effects of Ergosan on immune system of aquatic
animals, there is no data available on antioxidant
activities of Ergosan. The aim of the present study
was to investigate and evaluate the radical scavenging
activities of Ergosan extract by DPPH (1, 1-
diphenyl-2-picrylhydrazyl) free radical scavenging
assay, and the possible effects of gamma irradiation
on its assumed radical scavenging activities.
Materials and Methods: Ergosan was irradiated with
gamma rays (10, 20, 30, 40 and 50 kGy), and their
structural changes and antioxidant activities were
investigated by UV absorbanceand DPPH (1,1-
diphenyl-2-picrylhydrazyl) assays, respectively.
Results: The gamma irradiation decreased the
average pH of irradiated Ergosan, and UV spectra of
irradiated product showed increase in the number of
carboxyl groups and double bonds. Our results
showed that 30 kGy irradiated Ergosan suspension
had significant higher level of antioxidant activity in
comparison with non-irradiated Ergosan (P<0.05).
Also, the reducing power values of 30 and 50 kGy
irradiated Ergosan were higher than that of nonirradiated
(P<0.05) and the other doses of irradiation
couldn’t make any significant difference in reducing
power of Ergosan. Conclusion: Results indicate that
the 30 kGy irradiated Ergosan might be an appropriate
candidate for the use in aquatic animal diets as a
natural antioxidant agent besides its immunostimulant
role. Iran. J. Radiat. Res., 2012 9(4): 245-249
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| Keywords: Ergosan, gamma irradiation, antioxidant activity, reducing power |
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Full-Text [PDF 278 kb]
(2634 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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