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Comparison of radiosensitizing effect of Resveratrol on monolayer and spheroid culture of DU145 prostatic cell line
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M.S. Nezamtaheri   , S. Khoei , A.R. Nikoofar , B. Goliaei |
| , goliaei@ibb.ut.ac.ir |
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Abstract: (9876 Views) |
| Background: Radiotherapy is an established
therapeutic modality for prostate cancer. Resveratrol,
a natural antioxidant, has been shown to inhibit
carcinogenesis and to block the process of tumor
initiation and progression. No data is available on the
response of cellular spheroid to Reseveratol. In this
study we have examined the effect of Resveratol on
the radiation response of human prostate cell line
DU145 in monolayer and spheroid cultures. Materials
and Methods: Radiosensitivity was assessed using
viability and colony formation assay. Apoptosis and
necrosis were assessed using acridine orange/
ethidium bromide double staining. Results: The
colony formation assay did not show any significant
radio-sensitizing effect, but apoptosis assay showed
significant radio-sensitizing effect of Resveratol on
DU145 cells grown as monolayer. In the spheroid
cells the results of apoptosis test were not significant
and corresponded closely to the result of survival
curve. Conclusion: While Resveratol could sensitize
DU145 cells in monolayer to ionizing radiation, it did
not have any effect on sensitivity of cells cultured in
spheroid cultures. Iran. J. Radiat. Res., 2012 10(3‐4):
177‐181
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| Keywords: Resveratrol, X-ray irradiation, multicellular spheroid, radioresistance, apoptosis. |
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Full-Text [PDF 238 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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