%0 Journal Article %A S. Hosseini, %A H. Mozdarani, %T Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry %J International Journal of Radiation Research %V 2 %N 1 %U http://ijrr.com/article-1-36-en.html %R %D 2004 %K PCC, calyculin A, human lymphocytes, %X Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. Materials and Methods: Peripheral blood was obtained from a healthy donor and exposed to various doses (0.25–4 Gy) of γ -rays. The frequency of simple breaks and dicentrics were scored in G2/M chromosomes of Giemsa stained cells. Results: Results show that the frequency of simple chromosome breaks appears to increase linearly with dose while the frequency of dicentrics apparently increases linear-quadratically with the dose. Conclusion: Induction of chromosome condensation by calyculin A is a powerful biodisimetric method, which provides a high number of spreads for analysis. With the use of this method, it is possible to overcome problems related to low mitotic index or cell-cycle alterations in routine metaphase analysis and low fusion rate in conventional PCC technique. Iran . J. Radiat. Res., 2004 2 (1): 15-20 %> http://ijrr.com/article-1-36-en.pdf %P 15-20 %& 15 %! Induction of premature chromos %9 Original Research %L A-10-1-36 %+ %G eng %@ 2322-3243 %[ 2004