AU - Heshmati, E. AU - Mozdarani, H. AU - Abdolmaleki, P. AU - Khoshaman, K. TI - Radiosensitizing effects of gemcitabine on aerobic and chronically hypoxic HeLa and MRC5 cells in-vitro PT - JOURNAL ARTICLE TA - Int-J-Radiat-Res JN - Int-J-Radiat-Res VO - 10 VI - 1 IP - 1 4099 - http://ijrr.com/article-1-878-en.html 4100 - http://ijrr.com/article-1-878-en.pdf SO - Int-J-Radiat-Res 1 AB  - Background: Gemcitabine (2′, 2′-difluoro-2′- deoxycytidine, an analogue of deoxycytidine) is a relatively new drug with wide range of anti-cancer activity. In this study, radiosensitizing effects of gemcitabine was investigated on HeLa and MRC5 human originated cell lines under both chronically hypoxic and normoxic conditions using the micronucleus (MN) assay. Materials and Methods: For induction of chronic hypoxia, the cell culture flasks were saturated with N2 gas. To evaluate the radiosensitizing effects, in the presence of the non-genotoxic concentration (1ng/ml) of gemcitabine, cells were exposed to different doses (0.5, 1, 2 Gy) of X-ray in both chronically hypoxic and normoxic conditions. Results: Results showed that there was no significant difference in MN induction under chronically hypoxic and normoxic condition when using 1 ng/ml gemcitabine alone, however in the absence of drug, MN induction was significantly different in irradiated cells (P CP - IRAN IN - Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran LG - eng PB - Int-J-Radiat-Res PG - 11 PT - Original Research YR - 2012