AU - Yu, X.N. AU - Wang, M. TI - LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radio-sensitivity PT - JOURNAL ARTICLE TA - Int-J-Radiat-Res JN - Int-J-Radiat-Res VO - 20 VI - 1 IP - 1 4099 - http://ijrr.com/article-1-4060-en.html 4100 - http://ijrr.com/article-1-4060-en.pdf SO - Int-J-Radiat-Res 1 ABĀ  - Background: To investigate whether LncRNA LINC01204 affects lung cancer cell apoptosis, migration, invasion and radiosensitivity by regulating miR-214. Materials and Methods: Normal lung cells HBE and lung cancer cells A549 were cultured in-vitro, and pcDNA3.1, pcDNA3.1-LINC01204, pcDNA3.1-LINC01204 and miR-NC, pcDNA3.1-LINC01204 and miR-214 mimics were transfected into A549 cells, respectively. qRT-PCR, flow cytometry, scratch test, and Transwell chamber test, clone formation test, dual luciferase, and Western blot methods were used in this study. Results: Compared with HBE cells, A549 cells had significantly reduced LINC01204 expression level (P<0.05), and significantly increased miR-214 expression level (P<0.05); transfection of pcDNA3.1-LINC01204 could significantly increase apoptosis rate and Cleaved-caspase3, E-cadherin protein levels (P<0.05), reduce migration healing rate, N-cadherin protein levels and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 1.950, reduce the number of invasive cells (P<0.05). Dual luciferase report experiments confirmed that LINC01204 can bind to miR-214 in a targeted way; co-transfection of pcDNA3.1-LINC01204 and miR-214 mimics can significantly reduce the apoptosis rate and Cleaved-caspase3 and E-cadherin protein levels (P<0.05), improve the migration healing rate, N-cadherin protein level and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 0.728, increase the number of invasive cells (P<0.05). Conclusion: LINC01204 overexpression can negatively regulate miR-214 expression to promote lung cancer cell apoptosis and inhibit migration and invasion, thereby enhancing radiosensitivity. CP - IRAN IN - Department of Oncology, Weihai Central Hospital, Weihai, 264400, China LG - eng PB - Int-J-Radiat-Res PG - 15 PT - Original Research YR - 2022