International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1398
7
1
gregorian
2019
10
1
17
4
online
1
fulltext
en
Topically applied melatonin ameliorates radiation-induced skin fibrosis in mice
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<div style="text-align: justify;">Background: We investigated whether topical administration of melatonin ameliorates radiation-induced skin fibrosis (RISF) and inhibits the expression of profibrogenic genes in mice. Materials and Methods: Forty-eight female BALB/c mice were randomly divided into three groups: topically applied 5% ethanol (Control), topically applied 5% ethanol plus irradiation (IR), and topically applied melatonin plus irradiation (Mel+IR). The right hind legs of the IR and Mel+IR group mice were exposed to two fractions of electron beam radiation (20 Gy × 2). For 4 weeks, melatonin solution (10 mg/day) was topically applied to Mel+IR group mice. Fourteen days after IR, the relative levels of transforming growth factor (TGF)-β1 mRNA expression and TGF-β1 protein in skin specimens were analyzed by real-time quantitative PCR and immunohistochemical staining. Dermal thickness and tissue collagen accumulation were measured at 100 days post irradiation. Results: The Radiation caused a 2.2-fold increase in TGF-β1 mRNA expression relative to that in control group, which was decreased by 37% following melatonin treatment (P = 0.024). We also observed substantial reduction of TGF-β1 expression in immunohistochemical studies. The mean values of dermal thickness were 105 ± 11 μm (Control), 195 ± 21 μm (IR), and 148 ± 19 μm (Mel+IR). Dermal thickness and collagen accumulation, which increased in the IR group, was significantly reduced by topically applied melatonin. Conclusion: Topical administration of melatonin successfully attenuated RISF.</div>
Melatonin, Radiation, Fibrosis, Transforming growth factor-β, Topical application.
617
624
http://ijrr.com/browse.php?a_code=A-10-1-824&slc_lang=en&sid=1
D.H.
Kim
7900319475328460014009
7900319475328460014009
No
Department of Radiation Oncology, Otorhinolaryngology, and Hemato-oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Pusan National University, Busan, Korea
Y.M.
Choi
7900319475328460014010
7900319475328460014010
No
Department of Radiation Oncology, DongA University Hospital, Busan, Korea
Y.K.
Ki
7900319475328460014011
7900319475328460014011
No
Department of Radiation Oncology, Pusan National University Yangsan Hospital and Pusan National University School of Medicine, Yangsan, Korea
K.S.
Cho
7900319475328460014012
7900319475328460014012
No
Department of Radiation Oncology, Otorhinolaryngology, and Hemato-oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Pusan National University, Busan, Korea
Y.J.
Choi
7900319475328460014013
7900319475328460014013
No
Department of Radiation Oncology, Otorhinolaryngology, and Hemato-oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Pusan National University, Busan, Korea
W.T.
Kim
rokwt@hanmail.net
7900319475328460014014
7900319475328460014014
Yes
Department of Radiation Oncology, Otorhinolaryngology, and Hemato-oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Pusan National University, Busan, Korea