International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1386
6
1
gregorian
2007
9
1
5
2
online
1
fulltext
en
Reduced DNA damage in tumor spheroids compared to monolayer cultures exposed to ionizing radiation
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<strong>Background:</strong> Several cell lines when cultured under proper condition can form three dimensional structures called multicellular tumor spheroids. Tumor spheroids are valuable in vitro models for studying physical and biological behavior of real tumors. A number of previous studies using a variety of techniques have shown no relationship between radiosensitivity and DNA strand breaks in monolayer and spheroid model of cell culture. <strong>Materials and Methods:</strong> In the present study, the radiosensitivity of cells grown as monolayer and spheroid were measured with colony assay and the role of DNA strand breaks in this sensitivity was examined using single cell gel electrophoresis assay also known as Comet assay. <strong>Results:</strong> In the present experiment, spheroids showed more radioresistance than monolayers as judged by the number of colonies which they produced after radiation. Under the same experimental conditions, less level of DNA damage was detected in spheroids using "comet assay" technique. <strong>Conclusion:</strong> It was concluded that the loss of radioresistance which was observed in monolayer cultures might have been attributed to the higher level of DNA damage occurred in the cells.
Multicellular tumor spheroid, DU 145, radiosensitivity, DNA damage, comet assay.
63
69
http://ijrr.com/browse.php?a_code=A-10-1-235&slc_lang=en&sid=1
Gh.R.
Fazeli
79003194753284600779
79003194753284600779
No
S.
Khoei
79003194753284600780
79003194753284600780
No
A.R.
Nikoofar
79003194753284600781
79003194753284600781
No
B.
Goliaei
79003194753284600782
79003194753284600782
Yes