International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1399
10
1
gregorian
2021
1
1
19
1
online
1
fulltext
en
Induction of a bystander effect after therapeutic ultrasound exposure in human melanoma: In-vitro assay
Medical Physics
Medical Physics
تحقيق بديع
Original Research
<div style="text-align: justify;">Background: The induction of bystander effect via ionizing radiation has been well proven. However, few studies have investigated the bystander effect following non-ionizing radiation, such as ultrasound waves. Here, the bystander effect after different sonication times on human melanoma cell line (A375), is evaluated by assessing cell viability and apoptosis. Materials and Methods: The cells were divided into two main target and bystander groups. Target cells were exposed to 1 MHz ultrasound at 2 W/cm² intensity for 1, 2, 5 and 10 min with an ultrasound unit. Then, bystander cells received the cell culture medium of target cells. MTT and flow cytometry assays were used to determine the cell’ viability at different times after exposure and medium transfer, as well as the detection of apoptosis. Results: The cell’ viability in ultrasound-exposed target cells was less than 75% for 24 and 48 h incubation. Furthermore, bystander cell’ viability was not significantly different from the control group 1 and 12 h after receiving the culture medium of target cells. However, bystander cells viability 24 and 48 h after target cells medium transfer was significantly decreased (P=0.01). The apoptosis rate of bystander cells, 24 and 48 h after receiving the cell culture medium of target cells, showed significant differences from the control group. Conclusion: This research results revealed that the ultrasound waves could induce a biological effect in A375 bystander cells which were not directly exposed to direct ultrasound.</div>
Bystander effect, ultrasound waves, MTT assay, apoptosis assay, A375 cell line.
183
189
http://ijrr.com/browse.php?a_code=A-10-2055-100&slc_lang=en&sid=1
M.
Rezaei
7900319475328460020023
7900319475328460020023
No
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
R.
Kamran Samani
7900319475328460020024
7900319475328460020024
No
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
M.
Kazemi
7900319475328460020025
7900319475328460020025
No
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
A.
Shanei
shanei@med.mui.ac.ir
7900319475328460020026
7900319475328460020026
Yes
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
S.H.
Hejazi
7900319475328460020027
7900319475328460020027
No
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran