International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1400
7
1
gregorian
2021
10
1
19
4
online
1
fulltext
en
Radiation protection and cytotoxicity effects of different concentrations of cerium oxide nanoparticles in aqueous solution combined with sodium dodecyl sulphate in Vero cells irradiated with 18 MV beams
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<div style="text-align: justify;">Background: This study aimed to assess and compare the radioprotective and cytotoxic effects of various concentrations of cerium oxide nanoparticles (CONPs) in aqueous solution combined with sodium dodecyl sulphate (SDS) against high energy X-ray beams in Vero cells. Materials and Methods: The scanner electron microscopy (SEM) method was used to analyze the properties of CONPs. The cells were incubated with different concentrations of CONPs in aqueous solution combined with SDS. The non-toxic CONPs concentrations in Vero normal cells were determined using MTT assay. The cell’s uptake was measured by an UV/VIS absorption spectrophotometry. The cells were irradiated with different doses of 18 MV photon (1, 2, and 3 Gy), and their viabilities at various concentrations were measured to evaluate the radiation protection effects of CONPs. Results: The CONPs concentrations lower than 600 μg/ml were referred as non-toxic effects regarding MTT results. The 600 μg/ml was regarded as the highest radioprotection effect among the non-toxic concentrations (P-value˂0.05). The average percentage of cell viability improvement was estimated as 17, 23.61, and 27.21% for 1, 2, and 3 Gy doses, respectively, compared to the control group (with no CONPs). Pearson’s correlation coefficients between the CONPs concentration and cell viability were obtained as 0.96, 0.99, and 0.99 for 1, 2, and 3 Gy doses, respectively; showing that the increased concentration leads to an increase in higher radioprotection. Conclusion: The 600 μg/ml of CONPs aqueous solution combined with SDS, as a stable non-toxic concentration, has the highest radiation protection effect when exposed to high-energy photon beams. So, this concentration can be considered as an appropriate candidate of radioprotection for further research.</div>
Cerium oxide nanoparticles, sodium dodecyl sulphate, radiation protection, high photon energy, MTT test.
913
920
http://ijrr.com/browse.php?a_code=A-10-1-882&slc_lang=en&sid=1
Y.
Moghimi
7900319475328460019725
7900319475328460019725
No
Department of Medical Physics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
A.
Banaei
7900319475328460019726
7900319475328460019726
No
Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
M.
Majdaeen
Mehrsa.majdaeen@yahoo.com,
7900319475328460019727
7900319475328460019727
Yes
Department of Radiotherapy and Oncology, Razi Hospital, Rasht, Iran
H.
Zamani
7900319475328460019728
7900319475328460019728
No
Department of Medical Physics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
R.
Abedi-Firouzjah
7900319475328460019729
7900319475328460019729
No
Department of Medical Physics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran