International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1401
10
1
gregorian
2023
1
1
21
1
online
1
fulltext
en
Immunomodulatory effects of ionizing radiation on peripheral blood mononuclear cells
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-word"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="tr" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:tr">Avoiding exposure to ionizing radiation due to environmental factors is almost inevitable in daily life. Here, we aimed to investigate the possible immunomodulatory effects of ionizing radiation on NK and T cell activation using Peripheral Blood Mononuclear Cells (PBMC). We measured the pro-inflammatory cytokines INFγ, IL-2 and TNFα, as well as Granzyme B. In addition, we determined the expression levels of CD28, NKG2D (CD314) receptors, which play a key role in the activation of T and NK cells, respectively. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="tr" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:tr">20 ml peripheral blood samples were taken from healthy volunteer donors and exposed to radiation doses of 0, 1, 3 and 5 Gy. ELISA analysis was used to measure Granzyme B, INFy, TNFα and IL2. Expression of CD28, NKG2D (CD314) receptors was measured by qRT-PCR analysis. Apoptosis and necrosis were measured by AnnexinV/7AAD analysis. Catalase activity was measured using hemolysates from irradiated blood samples. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="tr" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:tr">Here we show that IR exposure induces necrotic cell death in PBMCs as the main response. IR exposure significantly induced secretions of Granzyme B, TNFα, IL2, and INFγ in a dose-dependent manner. In addition, mRNA levels of CD28 and NKG2D expressions were increased by 3 Gy IR exposure, but decreased by 5 Gy, while catalase activity increases with 1 Gy IR treatment, 3 and 5 Gy decreases. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusions</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="tr" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:tr">Our results suggest that not only high doses but even low doses of radiation can modulate the immune response through cytokine secretion and activation of T and NK cell receptors.</span></span></span></span></span></span></span></span></div>
Ionizing radiation, immune cells, necrotic cell death, CD28, NKG2D.
73
78
http://ijrr.com/browse.php?a_code=A-10-1-1007&slc_lang=en&sid=1
N.
Öztürk
7900319475328460023067
7900319475328460023067
No
Aydın Adnan Menderes University, Faculty of Medicine, Department of Radiation Oncology, Aytepe Campus, Aydin, Turkey
A.
Karlıtepe
7900319475328460023068
7900319475328460023068
No
Aydın Adnan Menderes University, Faculty of Medicine, Department of Medical Biology, Aytepe Campus, Aydin, Turkey
B.
Depboylu
7900319475328460023069
7900319475328460023069
No
Aydın Adnan Menderes University, Faculty of Medicine, Department of Radiation Oncology, Aytepe Campus, Aydin, Turkey
M.
Kılıç Eren
mkilic@adu.edu.tr
7900319475328460023070
7900319475328460023070
Yes
Aydın Adnan Menderes University, Faculty of Medicine, Department of Medical Biology, Aytepe Campus, Aydin, Turkey