en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">The existence of correlates between radiation and plasma metabolites in rats might be affected by feeding conditions. </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">The rats were kept without food and water for a certain time before the blood was harvested on the seventh day after X-ray irradiation at doses of 0 and 8 Gy. The plasma metabolites were tested using Enzyme-Linked Immunosorbent Assay (ELISA).</span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">Our results showed that abrosia for 2 h before blood harvesting could increase the level of detections of both interleukin-6 (IL-6) and glycine (Gly) in rats. Furthermore, abrosia and meanwhile water deprivation for 2-4 h increased better the level of detections of IL-6 and Gly in rats.</span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">The level of detections of biomarkers in the blood may be more authentic and can better reflect the changes in the experimental animals after stress when they are treated by both abrosia and water deprivation for 2 h before blood harvesting.</span></span></span></span></span></span></span></span></span></span></div> Abrosia, water deprivation, blood harvesting, level of detections of plasma metabolites, irradiation. 111 116 http://ijrr.com/browse.php?a_code=A-10-1-1013&slc_lang=en&sid=1 R. Li 7900319475328460023118 7900319475328460023118 No Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, China J. Cai 7900319475328460023119 7900319475328460023119 No Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, China Y. Wang 7900319475328460023120 7900319475328460023120 No Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, China C. Fu 7900319475328460023121 7900319475328460023121 No Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, China B. Hu brhu@wmu.edu.cn 7900319475328460023122 7900319475328460023122 No Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, China