International Journal of Radiation Research

en Cytoprotective effects of amifostine and melatonin against radiation-induced oral mucositis in rats Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Amifostine (AMI) protects against radiotherapy (RT)-induced toxicities and melatonin (MEL) is a potent free radical scavenger. This study was performed to investigate the protective effects of AMI and MEL on radiation-induced oral mucositis (ROM). Materials and Methods: Thirty female Sprague-Dawley rats were randomly divided into five groups as follows: the control (Cont), RT alone (RT), RT+AMI, RT+MEL, and RT+AMI+MEL. RT groups were irradiated with a single dose of 15 Gy to the head. AMI (200 mg/kg) and MEL (100 mg/kg) were administered intraperitoneally 1 hour before radiation exposure. Changes in body weights and histology in irradiated tongue tissues were analyzed 10 days after exposure. Results: AMI and/or MEL treatment significantly prevented irradiation-induced body weight loss and promoted epithelial cell proliferation. Mean epithelial thickness was markedly higher in the AMI+MEL group (73.9 ± 9.7 um) than in the RT group (28.8 ± 13.9 um) (P<0.001), and Ki67 expression was significantly higher in AMI, MEL, and AMI+MEL groups than in the RT group (p < 0.001). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) revealed that AMI+MEL treatment significantly inhibited radiation-induced apoptosis in irradiated epithelium (p=0.006). Conclusion: AMI and MEL administrations similarly protected animals from ROM and, when co-administered, had additive effects.</div> Amifostine, melatonin, radiation-induced oral mucositis, rats. 261 265 http://ijrr.com/browse.php?a_code=A-10-1-1036&slc_lang=en&sid=1 D. Park 7900319475328460023628 7900319475328460023628 No Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea D.H. Kim dh2372@pusan.ac.kr 7900319475328460023629 7900319475328460023629 Yes Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea W.T. Kim 7900319475328460023630 7900319475328460023630 No Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea J.H. Nam 7900319475328460023631 7900319475328460023631 No Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea D.C. Kim 7900319475328460023632 7900319475328460023632 No Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea K.H. Lee 7900319475328460023633 7900319475328460023633 No Department of Radiation Oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan, Republic of Korea Y.K. Ki 7900319475328460023634 7900319475328460023634 No Department of Radiation Oncology, Pusan National University Yangsan Hospital and Pusan National University School of Medicine, Yangsan, Republic of Korea J.H. Joo 7900319475328460023635 7900319475328460023635 No Department of Radiation Oncology, Pusan National University Yangsan Hospital and Pusan National University School of Medicine, Yangsan, Republic of Korea H.S. Jeon 7900319475328460023636 7900319475328460023636 No Department of Radiation Oncology, Pusan National University Yangsan Hospital and Pusan National University School of Medicine, Yangsan, Republic of Korea