en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The Background: We evaluate the effects of radiofrequency electromagnetic field (RF-EMF) on rat brain and testicular tissue using histopathology, comet assay, and real-time quantitative PCR techniques. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Two equal groups of fourteen rats one for sham-control and the other for exposure (n = seven) were created. For a duration of 14 days, the exposure group (2100 MHz, testicular tissue SAR values of 163 mW/kg for 10 g, brain tissue SAR values of 292 mW/kg on average) was subjected to five hours of exposure per day. Evaluations were conducted on tissue gene expression levels, histopathology, and DNA damage to brain tissue. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The histological examination of brain tissue from the exposed group revealed vascular alterations and significant edema (p < 0.05). It was determined that RF radiation-induced much more cellular damage in the exposed group (18.26% tail DNA) than in the control group (4.06% tail DNA). Signs of deterioration in spermatogenic cells in the testicular tissue of the exposed group also changed significantly (p < 0.05). The </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="font-style:italic"><span style="language:en-US">Bax </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">and </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="font-style:italic"><span style="language:en-US">bcl-2 </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">genes showed a significant difference (p < 0.05) in the mRNA level data, whereas the </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="font-style:italic"><span style="language:en-US">p53</span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US"> genes showed no significant change (p > 0.05). </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">These findings suggest that it may cause some histopathological and cellular damage in brain and testis tissue.</span></span></span></span></span></span></span></span></span></div> 2100 MHz, Brain, Radiation, Histopathological, qRT-PCR, Radiofrequency, Testicular. 529 536 http://ijrr.com/browse.php?a_code=A-10-1-1205&slc_lang=en&sid=1 M.C. Yavas mcihanyavas@artuklu.edu.tr 7900319475328460027867 7900319475328460027867 Yes Department of Biophysics, Faculty of Medicine, Mardin Artuklu University, Mardin, Turkey A. Kilitci 7900319475328460027868 7900319475328460027868 No Department of Pathology, Faculty of Medicine, Düzce University, Düzce, Turkey E. Çelik 7900319475328460027869 7900319475328460027869 No Department of Medical Biology, Faculty of Medicine, Kırşehir Ahi Evran University, Kırşehir, Turkey K. Yegin 7900319475328460027870 7900319475328460027870 No Department of Electrical and Electronics Engineering, Ege University, Izmir, Turkey B. Sirav 7900319475328460027871 7900319475328460027871 No Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey S. Varol 7900319475328460027872 7900319475328460027872 No Department of Biophysics, Faculty of Medicine, Erciyes University, Kayseri, Turkey