International Journal of Radiation Research

en Assessment of chromosomal aberrations induced in patients undergoing vertebroplasty and workers occupationally exposed to low-dose ionizing radiation Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: The Chromosomal aberrations (CAs) are among the most crucial biological effects resulting from ionizing radiation. The aim of the current study is to investigate cytogenetic effects after both short- and long-term exposure of low irradiation doses (less than100 mSv). Materials and Methods: In this study, two groups were included: 1) eighteen patients (age 54 - 90 years) undergoing fluoroscopic X-ray guided vertebroplasty for a short time (0.63 - 2.07 min) and 2) eighteen workers (age 40 - 66 years) employed in Bulgarian Nuclear Power Plant (NPP) (19 - 42 working years). Blood samples were taken from all subjects followed by culturing in RPMI-1640 medium. After cells harvesting and standard samples processing, chromosomal aberrations frequency was analyzed in Giemsa stained metaphase spreads. For patients group, blood samples were collected before and after the medical procedure. Nine healthy volunteers (from NPP administrative staff) served as reference controls in workers group. Results: The mean frequency of total CAs after vertebroplasty (0.0213 ± 0.0016 per cell) is slightly but significantly increased compared to the baseline level before the medical procedure (0.0143 ± 0.0013; P = 0.013). However, variabilities at the individual level were found. In the workers group, the total CAs yield raised 4 times relative to controls (0.0113 ± 0.0017 vs. 0.0028 ± 0.0012; P = 0.001). At the same time, no correlation was found between aberrations frequency and accumulated dose in both patients and workers. Conclusion: Low doses exposure whether acute or chronic results in deoxyribonucleic acid (DNA) damages and consequent CAs.</div> Low-dose irradiation, chromosomal aberrations, vertebroplasty, NPP workers. 63 68 http://ijrr.com/browse.php?a_code=A-10-1-1456&slc_lang=en&sid=1 N. Kostova 7900319475328460032737 7900319475328460032737 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria A. Staynova 7900319475328460032738 7900319475328460032738 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria L. Popova-Hadjiiska 7900319475328460032739 7900319475328460032739 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria D. Georgieva 7900319475328460032740 7900319475328460032740 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria H. Hristov 7900319475328460032741 7900319475328460032741 No Department of Neurosurgery, “St. Ivan Rilski” University Hospital, Medical University, Sofia, Bulgaria M. Atanasova 7900319475328460032742 7900319475328460032742 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria I. Ivanova 7900319475328460032743 7900319475328460032743 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria E. Zaharieva 7900319475328460032744 7900319475328460032744 No Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria H. Tsonev 7900319475328460032745 7900319475328460032745 No Department of Neurosurgery, “St. Ivan Rilski” University Hospital, Medical University, Sofia, Bulgaria R. Hristova r.hristova@ncrrp.org 7900319475328460032746 7900319475328460032746 Yes Department of Radiobiology, National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria