en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Irradiation at the high dose may attenuate renal hemodynamics while retaining the therapeutic effect on cancer cells and thus can life-threatening. Hence the effects on kidneys of irradiation become increasingly crucial. This study aimed to examine oxidative stress, inflammatory response, vascular injury, apoptotis and deoxyribonucleic acid (DNA)DNA damage induced acute high dose ionising radiation in rat kidney and to investigate whether umbelliferone (UMB) has protective effect on radiation-induced renal damage and dysfunction. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Materials and Methods: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Rats were randomly allocated into eight groups (n = 7): the control group, given physiological saline; the model group, exposured radiation (whole body irradiated with single high dose of 12 Gy of gamma rays) to induce radiation damage in renal tissue; UMB groups, received UMB (30, 60 and 90 mg/kg) alone and treatment groupsreceived orally UMB for 5 days before radiation exposure. Animals were divided groups; control,&nbsp; group whole body irradiated with single dose 12 Gy, groups receiving umbelliferone alone and groups receiving umbelliferone for 5 days before irradiated. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Results: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">Radiation-induced pathological findings and functional disorders were significantly ameliorated in UMBumbelliferone-treated groups. Our results revealed that umbelliferone pretreatment in the kidney plays an important role in the regulation of blood pressure in irradiation-induced renal injury. UMB increases the antioxidant activity and decreases inflammatory response in kidney tissue against radiation toxicity. Notably, it exhibits anti-apoptotic and anti-genotoxic actions in damaged renal cells. It also ameliorates the blood urea nitrogen (BUN) and creatinine values. </span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Conclusion: </span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">In conclusion, UMBumbelliferone from coumarin compounds is a targeted and promising drug to prevent kidney necrosis and dysfunction due to radiation therapy.</span></span></span></span></span></span></span></span></span></span></div> 8-hydroxy-2'-deoxyguanosine (8-OHdG)DNA damage, ıonizing Rradiation, renal dysfunctioninjury, umbelliferone, thromboxane B2 (TXB2)Vascular injury. 115 122 http://ijrr.com/browse.php?a_code=A-10-1-1464&slc_lang=en&sid=1 F. Geyikoglu 7900319475328460032862 7900319475328460032862 No Faculty of Sciences, Biology Deparment, Atatürk University, 25240 Erzurum, Turkey G.Y. Deniz gulsah.ydeniz@gmail.com 7900319475328460032863 7900319475328460032863 No Vocational School of Healh Services, Ataturk University, 25240 Erzurum, Turkey M.N.G. Demir 7900319475328460032864 7900319475328460032864 No Faculty of Sciences, Biology Deparment, Atatürk University, 25240 Erzurum, Turkey M. Arslan 7900319475328460032865 7900319475328460032865 No Faculty of Sciences, Biology Deparment, Atatürk University, 25240 Erzurum, Turkey B. Erdemci 7900319475328460032866 7900319475328460032866 No Faculty of Medicine, Radiation Oncology Department, Ataturk University, Erzurum, Turkey