en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-GB">To investigate the clinical value of combining the expression of circular RNA ubiquitin associated protein 2 (circRNA-UBAP2) and chemokine receptor 4 (CXCR4) messenger RNA</span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">（</span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">mRNA</span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="color:black"><span style="language:en-US">） </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">with endoscopic ultrasonography for the diagnosis of colorectal cancer (CRC). </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">A retrospective study was conducted. 104 patients with CRC and 87 patients with colorectal adenoma who were admitted to the hospital from May 2022 to May 2024 were enrolled in the study. The expression levels of circRNA-UBAP2 and CXCR4 mRNA in samples were detected. The relationship between the expression of circRNA-UBAP2 and CXCR4 mRNA and clinicopathological features of patients with CRC was analyzed, and the diagnostic efficacy of circRNA-UBAP2 and CXCR4 mRNA combined with endoscopic ultrasonography for CRC were evaluated. </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">The expression levels of circRNA-UBAP2 and CXCR4 mRNA in cancer tissues of patients with CRC were higher than those in adjacent tissues (P<0.05). Statistically significant differences in the expression levels of circRNA-UBAP2 and CXCR4 mRNA were observed among patients with different differentiation degrees, TNM stages, and lymph node metastasis status (P<0.05). The accuracy of endoscopic ultrasonography in diagnosing CRC was 87.96% (kappa value=0.758). The AUC (95% CI) of joint diagnosis was 0.980 (0.958-1.000), which was larger than that of separate diagnosis (P<0.05). </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:black"><span style="language:en-US">circRNA-UBAP2 and CXCR4 mRNA are highly expressed in patients with CRC, and are associated with differentiation degree, TNM staging, and lymph node metastasis. The two are helpful in distinguishing CRC from colorectal adenoma, and their combination with endoscopic ultrasonography can improve diagnostic efficacy.</span></span></span></span></span></span></span></span></span></span></div> Colorectal cancer, endoscopic ultrasonography, ubiquitin associated protein 2, chemokine receptor 4. 123 128 http://ijrr.com/browse.php?a_code=A-10-1-1465&slc_lang=en&sid=1 J. Duan 7900319475328460032868 7900319475328460032868 No Gastroenterology, Beihua University Affiliated Hospital, Jilin, Jilin Province, China Z. Gao 7900319475328460032869 7900319475328460032869 No Operation Room, Huadian People’s Hospital, Jilin, Jilin Province, China Z. Yang 7900319475328460032870 7900319475328460032870 No Gastroenterology, Beihua University Affiliated Hospital, Jilin, Jilin Province, China W. Huang 7900319475328460032871 7900319475328460032871 No Gastroenterology, Beihua University Affiliated Hospital, Jilin, Jilin Province, China X. Guan 7900319475328460032872 7900319475328460032872 No Gastroenterology, Beihua University Affiliated Hospital, Jilin, Jilin Province, China Y. Wang 15043210800@163.com 7900319475328460032873 7900319475328460032873 Yes Gastroenterology, Beihua University Affiliated Hospital, Jilin, Jilin Province, China