79 2322-3243 Dr. Hossein Mozdarani 5844 Radiation Biology The effects of radiofrequency ablation on lymphocytes, subsets and cytokines in patients with thyroid cancer: A meta-analysis Miao B. b Ruan G. c Meng F. d Zhang Q. e Pei J. f Li D. g b Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China c Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China d Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China e Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China f Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China g Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China 1 1 2025 23 1 1 12 21 01 2025 21 01 2025 Background: To systematically evaluate the effects of radiofrequency ablation on lymphocytes, subsets, and cytokines in patients with thyroid cancer. Materials and Method: Radiofrequency ablation, thyroid cancer, lymphocytes, and other key terms are used to search for randomized controlled clinical trials (RCTs) related to them.The data analysis is conducted using RevMan 5.3 software. The relative risk (RR) is used to analyze the effect magnitude of binary variable data. The mean difference (MD) represents the continuous variable data results. The interval estimation is presented as 95% CI. Results: The standardized mean difference between the CD3+, CD4+, sub-population counts, and CD4+/CD8+ratios among the studies was 1.91 (95% CI0.91 ~ 2.91), 0.07(95% CI-0.07 ~ 0.22). The combined effect quantity indicated that radiofrequency ablation had a significant effect on improving lymphocytes and sub-populations in thyroid cancer patients.The heterogeneity test results among different studies were I2 = 99%, 99%, P < 0.01 and 0.32. The results indicated a high level of heterogeneity between the two groups. The standardized mean difference of cytokine levels such as IL-6 and TNF-a in different studies was -4.20 (95% CI-4.30 ~ -4.10), -6.40 (95% CI-7.07 ~ -5.77). The combined effect quantity indicated that radiofrequency ablation had a significant effect on reducing serum inflammatory cytokine levels in thyroid cancer patients.The heterogeneity test results between different studies were I2 = 100%, 97%, and P < 0.01. The results indicated a high level of heterogeneity between the two groups. The differences between groups are statistically significant (x2 = 4.48, P < 0.01). Conclusion: Compared with other surgeries, radiofrequency ablation can inhibit CD4+, promote CD8+lymphocyte proliferation, and reduce inflammatory factors in thyroid cancer patients.
5856 Radiation Biology Dosimetric evaluation of brain radiotherapy using custom-made Rhizophora head phantom – comparison between Monte Carlo GATE and treatment planning system MONACO Zuber S.H. h Abdul Hadi M.F.R. i Hashikin N.A.A. j Samson D.O. k Ishak N.H. l Raof N.A. m Abdul Aziz M.Z. n Yusof M.F.M. o Rabaiee N.A. p h Centre for Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia i School of Physics, Universiti Sains Malaysia, Penang, Malaysia j School of Physics, Universiti Sains Malaysia, Penang, Malaysia k Department of Physics, Faculty of Science, University of Abuja, Abuja, Malaysia l Advanced Medical & Dental Institute, Universiti Sains Malaysia, Penang, Malaysia m Advanced Medical & Dental Institute, Universiti Sains Malaysia, Penang, Malaysia n Advanced Medical & Dental Institute, Universiti Sains Malaysia, Penang, Malaysia o Faculty of Health and Life Sciences, Management & Science University, Selangor, Malaysia p Department of Radiology, Kulliyyah of Medicine, International Islamic University Malaysia, Gombak, Malaysia 1 1 2025 23 1 13 20 25 01 2025 21 01 2025 Background: A custom-built head phantom has the potential as a dosimetric phantom in brain radiotherapy. Materials and Methods: Computed tomography (CT) was used to scan the phantom with CT data imported to treatment planning system (TPS) for dose computation. The dose prescription was pre-determined to deliver 300 cGy per fraction to the isocenter of the beam. 6 MV photon with parallel-opposed technique was used in this work. Absorbed dose computed in planning target volume (PTV) and organ at risks (OARs) were computed, and comparison was made with Monte Carlo (MC) Geant4 Application for Tomographic Emission (GATE). Results: The result demonstrates a close proximity of computed dose for TPS and GATE with a 1.6 % discrepancy for the PTV, with less than 1 % statistical uncertainty for GATE. The dose measured by TPS for most of the target of interest was in close proximity with the dose measured in MC. Conclusion: This study revealed the potential of MC GATE simulation in the dosimetric evaluation and verification of the treatment planning. 5894 Radiation Biology Non-ionization radiation hazard: Effect of mobile phone use on human cognitive functions in data exchange mode Heydari F. Yoosefee S. Khalili P. Ayoobi F. Shafiei S.A. Qom University of Medical Sciences, Qom, Iran Neuroscience Research Center, Spiritual Health Research Center, Qom University of Medical Sciences, Qom, Iran Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Occupational Safety and Health Research Center, NICICO, World Safety Organization and Rafsanjan University of Medical Sciences, Rafsanjan, Iran Neuroscience Research Center, Qom University of Medical Sciences, Qom, Iran 1 1 2025 23 1 21 27 09 02 2025 21 01 2025 Background: The Cell phones are a major part of people's lives in contemporary societies. Might their radiation be able to affect some cognitive functions while people drive? This study aims to investigate the effect of cell phone radiation on the brain’s cognitive functions. Materials and Method: Forty female students without depression or anxiety volunteered in the cross-sectional study. During one session, the volunteers were randomly exposed to cell phone radiation (20 participants in the first and 20 participants in the second). Participants performed four cognitive tests in each session. A p-value of less than 0.05 was taken as the cut-off point to consider a statistically significant result. Results: In the congruent part of the Stroop test, the reaction time (RT) was reduced in both groups during the time volunteers were exposed to radiation and there were significant differences between sessions in both groups (P=0.005 and P<0.001). These differences were significant between the two groups in different sessions. However, the number of errors decreased during exposure to radiation and this difference was significant in the first group (P=0.015). In the incongruent part of the Stroop test, the treatment showed that the radiation of mobile phones had a significant effect on the reduction of RT (P<0.001). Conclusion: Based on this study, it seems that cell phone radiation waves have a limited effect on RT, cognitive and executive function. Therefore, traffic accidents that occur during a mobile phone conversation might be solely due to the division of attention rather than a direct effect of cell phone waves. 5918 Radiation Biology An investigation of radon and heavy metal detection for cancer patients in Barji village, in the Iraqi Kurdistan region Kareem I.M. Umer M.I. Albrifkany M-T.S. Karim N.A. Fatah M.Y. Abdo H.R. Civil and Environmental Engineering college, University of Zakho, Zakho City, Kurdistan Region, Iraq Soil & Water Department, College of Agricultural Science Engineering, University of Dohuk, Duhok City, Kurdistan Region, Iraq Duhok Environmental Directorate, Duhok City, Kurdistan Region, Iraq Duhok Environmental Directorate, Duhok City, Kurdistan Region, Iraq Duhok Environmental Directorate, Duhok City, Kurdistan Region, Iraq 1 1 2025 23 1 29 36 16 02 2025 21 01 2025 Background: Exposure to radiological agents such as natural radon gas and consumption of heavy metals in edible plants are two potential causes of unusual cancer in Berje village near Amedi town in Duhok city in the Iraqi Kurdistan Region. Material and Methods: Samples of soil, fruits, and vegetables were collected at various stages in the village, particularly at locations close to the homes of cancer patients. The collected samples underwent digestion and heavy metal absorption tests to detect ratio of heavy metal content as well as in their contents of radon. Results: showed that both heavy metals and radon gas in all samples were with different ratios. Moreover, results revealed that the evolution of radon ranged between 51.375 and 170.25 Bq.m-3, which is within the normal range of radon emission for soil sample. Similarly, results demonstrated that the average content of radon ranged between 0 and 24.1 Bq/kg in most fruits and vegetables which are within the allowable range. However, cadmium was found to be in marginal limits as target hazard quotient (THQ) for lead was greater than 1. Furthermore, bioaccumulation factors of tested fruits and vegetables were less than 1. Conclusion: The translocation of radon from soil to plants is only hazardous for tomatoes and common purslane. Furthermore, health risk index (HRI) of lead and cadmium was between 1 and 5, which indicates a threat to human health. The hazardous index (HI) was also larger than 1, which implies that heavy metals had significant non-carcinogenic effects on human health. The study found that the target cancer risk (TCR) of lead was low as results demonstrated that heavy metal content was within allowable range. 5919 Radiation Biology Cytogenetic and cytotoxic effects of melatonin and saffron on lymphocytes of luminal A and luminal B breast cancer patients irradiated in vitro Mozdarani H. Mozdarani S. Pakniyat F. Mozdarani S. Nosrati H. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Cytogenetics, Cytogenome Medical Genetics laboratory, Ale-Ahmad High way, Chamran Medical Building, Tehran, Iran Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Cytogenetics, Cytogenome Medical Genetics laboratory, Ale-Ahmad High way, Chamran Medical Building, Tehran, Iran Cancer Institute, Department of Radiotherapy, Tehran, Iran 1 1 2025 23 1 37 43 16 02 2025 21 01 2025 Background: Due to high radiation- sensitivity of breast cancer (BC) patients, use of radioprotectors to ameliorate the deleterious effects of radiation could be a priority. This study aimed to evaluate the reducing effects of saffron and melatonin on ionizing radiation-induced damages in lymphocytes of luminal A & B BC patients using cytome assay. Materials and Methods: Whole blood samples were collected from BC patients as well as healthy individuals. Cells were treated with either melatonin or saffron two hours prior to irradiation of G0 lymphocytes using a 6 MV linear accelerator, at a dose of 3Gy. Thirty-six hours after PHA stimulation, Cytochalasin-B was added to the cultures. Cell harvesting and staining was performed using standard method. 1000 binucleate cells were scored per sample for the frequency of micronuclei (MN) or apoptosis under a 1000x light microscope. Results: The frequency of background MN was significantly lower in normal individuals compared to BC patients. Pretreatments of "saffron and melatonin" alone made no significant changes in the frequency of MN in all groups. However, after 3Gy-irradiation the frequency of MN elevated dramatically (p<0.01). Pretreatment of lymphocytes with melatonin and saffron led to a decrease in the frequency of MN in all treatment groups (p<0.05). In terms of apoptosis induction, only melatonin was found to exert reducing effect on radiation induced apoptosis. No radioprotection was observed for saffron in lymphocytes of BC patients. Conclusion: Obtained results elucidated more pronounced role of melatonin in decreasing MN and apoptosis frequencies post irradiation in lymphocytes of BC patients. 5942 Radiation Biology Cardiac oxidative stress induced by cell phone electromagnetic radiation and the cardioprotective effect of aerobic exercise in rats Akbari H.A. Gaeini A.A. Kordi M.R. A.A. Ravasi A.A. Ravasi Department of Exercise Physiology, Faculty of Sports Sciences and Health, University of Tehran, Iran Department of Exercise Physiology, Faculty of Sports Sciences and Health, University of Tehran, Iran Department of Exercise Physiology, Faculty of Sports Sciences and Health, University of Tehran, Iran Department of Exercise Physiology, Faculty of Sports Sciences and Health, University of Tehran, Iran 1 1 2025 23 1 45 52 22 02 2025 21 01 2025 Background: Oxidative stress-induced tissue damage is a recognized concern linked to electromagnetic radiation (EMR) waves. Materials and Methods: In the present study, we explored the effect of moderate aerobic exercise (AE) on oxidative stress (OS) and cardiomyocyte damage resulting from cell phone EMR in rats. A total of 32 male Wistar rats were randomly allocated into the following groups: control (C), EMR, AE, and EMR+AE. Both EMR+AE and EMR groups were subjected to three hours of daily cell phone EMR (0.9-1.8 GHz) exposure for six weeks, while the AE and EMR+AE groups completed a treadmill AE five days per week. The heart was collected 48 hours after the last AE session to assess OS, histopathological damage, and left ventricular structure. Results: Tissue sections revealed that the EMR group had more significant heart tissue damage (increased apoptotic cells and collagen deposition) than the C group, and adding AE mitigated the damage (P<0.05). In the EMR group, in comparison to the EMR+AE, C, and AE groups, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) significantly decreased, and the concentration of malondialdehyde (MDA) elevated notably (P < 0.05). Exercise training in both the AE and EMR+AE groups resulted in a notable rise in SOD, CAT, and GPX activity, alongside a notable reduction in MDA concentration in comparison to the EMR group (P < 0.05). Conclusions: AE is advantageous in mitigating the adverse impacts of EMR emitted from mobile phones on the heart. 5943 Radiation Biology Assessment of indoor radon, radon exhalation rate, and radium levels within Kpando Municipality, Volta Region, Ghana Amable A.S.K. Otoo F. Buah-Bassuah P.K. Twum A. Manso E.N. Adeti J.P. Ahiave E. School of Basic and Biomedical Sciences, Department of Basic Sciences, University of Health and Allied Sciences, PMB 31, Ho-Volta, Ghana Radiation Protection Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana School of Physical Sciences, Department of Physics, University of Cape Coast, Cape Coast, Ghana School of Physical Sciences, Department of Physics, University of Cape Coast, Cape Coast, Ghana University for Development Studies, Department of Medical Imaging, Tamale, Ghana Nuclear Chemistry and Environmental Research Centre, Ghana Atomic Energy Commission, Ghana Ho Technical University, Department of Mathematics and Statistics, Ho, Ghana 1 1 2025 23 1 53 60 22 02 2025 21 01 2025 Background: Radium, a naturally occurring radioactive substance, and its decay product radon, a radioactive gas, can accumulate in the environment and pose health risks to residents. This study aims to assess levels of radium and radon in soils and dwellings in Kpando Municipality and its potential health risks, ensuring informed decision-making for public safety and environmental protection. Materials and Methods: Fifty-one soil samples were collected from seventeen (17) different locations within the Kpando Municipality. The samples were prepared and analyzed for radium concentrations using gamma-ray spectrometry and radon exhalation using closed-can techniques and CR-39 detectors. Radon gas concentrations were measured in one hundred and twenty (120) selected dwellings using CR–39 detectors. Results: The activity concentration of radium, radon levels in dwellings and radon exhalation in soil ranged from 3.4 to 48.1 Bq/kg (23.6 ±4.5 Bq/kg), 23.5 to 124.7 Bq/m3 (60.5±3.2 Bq/m3), and 1.3 to 13.3 µBq/m2h (8.1±0.5 µBq/m2h), respectively. Strong and weak positive correlations were observed between radium/radon exhalation (0.9) and radium/indoor radon (0.3). Radon levels in dwellings and soil exceeded WHO and UNSCEAR recommendations by 11 % and 14 %, respectively. However, 98 % of estimated annual effective doses were below UNSCEAR and ICRP reference levels. Conclusion: The mean activity concentrations of radium and radon were less than WHO and UNSCEAR action levels. Elevated radium and radon concentrations in certain dwellings pose health risks. Urgent measures are needed: further investigations, improved building design, ventilation and awareness campaigns to reduce exposure. 5946 Radiation Biology Clinical analysis of gamma knife radiosurgery for pituitary adenomas Shan J. Wen Y. Guo Y. Special Examination Department, Shandong Daizhuang Hospital, JiNing City, Shandong, China Lingcheng District Hospital of Traditional Chinese Medicine, Dezhou City, Shandong, China Gamma knife centre, Shandong Daizhuang Hospital, JiNing city, Shandong, China 1 1 2025 23 1 61 68 25 02 2025 21 01 2025 To evaluate the efficacy and safety of Gamma Knife radiosurgery in the treatment of pituitary adenomas. Materials and Methods: A retrospective analysis was conducted on 123 patients with pituitary adenomas who underwent Gamma Knife radiosurgery at Shandong Daizhuang hospital. All patients were followed up for 12 months post-treatment and complete follow-up data were obtained. Follow-up data included records of disease progression (medical history and physical examination changes), imaging and endocrine follow-up results at re-examination. Univariate analysis was performed to identify factors affecting tumor control and post-treatment complications, and factors with statistical significance (P <0.05) were subjected to multivariate analysis. Results: Clinical data of 123 patients with pituitary adenomas were included in this study, with 51 patients in the postoperative group who had previously undergone surgical treatment, and 72 patients who were treated first with Gamma Knife radiosurgery. Except for age, there were no statistically significant differences in baseline data between the two groups. Tumor volume [P=0.034, odds ratio (OR)=1.071, 95% confidence interval (CI): 0.121-5.206] and tumor invasion (P=0.005, OR=0.233, 95% CI: 0.202-0.392) were predictive factors for tumor progression. The results of the 1-year follow-up showed no statistically significant difference in efficacy between patients with pituitary adenomas who had previously undergone surgical treatment and those who were first treated with Gamma Knife radiosurgery. Conclusion: Gamma Knife radiosurgery is an effective treatment option for patients with pituitary adenomas, regardless of whether they have previously undergone surgical treatment. 5947 Radiation Biology Usefulness of cast-type bolus produced by 3D printing for photon beam treatment of primary cutaneous lymphoma: A phantom experiment Won Y. Kim S. Department of Radiation Oncology, Uijeongbu Eulji Medical Center, Eulji University, Gyeonggi-do, South Korea Department of Radiological Science, Gachon University Medical Campus, Namdong-gu, Incheon, South Korea 1 1 2025 23 1 69 75 25 02 2025 21 01 2025 Background: To examine the usefulness of photon beam radiation therapy for primary cutaneous lymphoma, a phantom experiment was conducted by fabricating and applying an M3 casting type customized bolus by three-dimensional (3D) printing. Materials and Methods: Several 3D printers were used to compare output times according to division to reduce the output time of cast-type bolus. The dose distribution and dose verification for each treatment plan of electron beam (6 MeV, 9 MeV) and photon beams (AP/PA, field-in-field 3D conformal radiation therapy [3D CRT], intensity modulated radiotherapy [IMRT]) were analyzed. During photon beam treatment, the inside of the molded bolus was filled with rice and M3 for the experiment. Results: Compared with the infill type, the split casting type output method improved the output speed of the 3D printer by up to 94.7% and could be applied to patients within 48 h. Moreover, the treatment plan of the photon beam, compared with that of the 9 MeV electron beam, improved the radical dose homogeneity index (rDHI) by 23.0% to 71.3% and the moderate homogeneity index (mDHI) by 7.5% to 18.6% in the planning tumor volume, indicating a more uniform dose distribution. Conclusion: The difference in treatment plan evaluations between M3 and rice was similar, but in dose delivery, the maximum errors were 3.1% and 6.5%, respectively, indicating that M3 was superior to rice. 5948 Radiation Biology Application value of low-dose computed tomography combined with serum tumor markers in diagnosis of early non-small cell lung cancer Xu R. Xu W. Liu D. Pan S. Zhu J. Shen Y. Wang C. Shen H. Department of Oncology,Affiliated People's Hospital of Jiangsu University, Zhenjiang City, jiangsu, Province China Department of Oncology,Affiliated People's Hospital of Jiangsu University, Zhenjiang City, jiangsu, Province China Department of Thoracic Surgery, Shandong Public Health Clinical Center (Shandong Provincial Chest Hospital), Shandong Province, China Department of Laboratory Medicine, Shengli Oilfield Central Hospital, Shandong Province, China Department of Thoracic Surgery, Shandong Public Health Clinical Center (Shandong Provincial Chest Hospital), Shandong Province, China Department of Respiratory Medicine, Shandong Public Health Clinical Center (Shandong Provincial Chest Hospital), Shandong Province, China Department of Thoracic Surgery, Shandong Public Health Clinical Center (Shandong Provincial Chest Hospital), Shandong Province, China Department of Oncology,Affiliated People's Hospital of Jiangsu University, Zhenjiang City, jiangsu, Province China 1 1 2025 23 1 77 81 25 02 2025 21 01 2025 Background: We aimed to explore the application value of low-dose computed tomography (LDCT) combined with serum tumor markers in the diagnosis of early non-small cell lung cancer (NSCLC). Materials and Methods: The clinical data of 176 patients suspected of early NSCLC screened from March 2017 to April 2022 were retrospectively collected. The diagnostic values of LDCT, serum tumor markers, and LDCT combined with serum tumor markers for early NSCLC were explored. Results: There were diverse imaging characteristics of early NSCLC, dominated by solid lesions, followed by part-solid nodules, but non-solid nodules were rare. The levels of serum carcinoembryonic antigen, cytokeratin 19 fragment antigen 21-1 and neuron specific enolase in patients with early NSCLC were higher than those in patients with benign lesions (P<0.05). LDCT combined with serum tumor markers had a higher Kappa coefficient than LDCT and serum tumor markers, indicating that it had the highest consistency with pathological diagnosis results (P<0.05). Both the sensitivity and accuracy of LDCT combined with serum tumor markers were higher than those of LDCT and serum tumor markers (P<0.05). Conclusion: LDCT combined with serum tumor markers can significantly improve the diagnostic sensitivity and accuracy for early NSCLC and the consistency with pathological diagnosis results, and effectively reduce misdiagnosis and missed diagnosis. 5952 Radiation Biology Physical aspects of skin dose distribution in tomotherapy of breast cancer Saadatmand P. Mahdavi S.R. Nikoofar A. Esmaili G. Jalilifar M. Khazaie S. Vejdani S. Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Radiation Oncology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Radiotherapy Department, Pars Hospital, Tehran, Iran Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Radiation Oncology, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran 1 1 2025 23 1 83 90 26 02 2025 21 01 2025 Background: Estimating the accuracy of Treatment Planning Systems (TPS) in skin dose calculation is essential to achieving the intended therapeutic outcomes in breast cancer radiotherapy. This study aims to validate tomotherapy TPS accuracy in skin dose estimation. Materials and Methods: The ability of Accuray Precision TPS to provide precise skin dose calculations was examined by utilizing the Gafchromic EBT3 film, which was placed on the surface of the cylinder Delta4 phantom. The target volume received a 2Gy dose following setup validation. The accuracy of TPS was assessed using distinct spatial resolutions for dose calculation in helical and direct Tomotherapy plans. Using the RIT software, gamma analysis was employed to evaluate the precision of TPS skin dose distribution relative to the EBT3 film. Results: Comparison of skin dose distribution between the TPS and EBT3 films demonstrated acceptable gamma passing rates for helical (up to 98.51%) and direct plan (up to 90.41%) using gamma index criteria of 5 mm/5%. However, the gamma index of helical and direct tomotherapy plans with passing criteria of 3 mm/3% was 84.15% and 79.12%, respectively. Our findings indicate satisfactory consistency (3-5%) between measured and calculated skin doses using the EBT3 film and TPS, employing "high" spatial resolution dose calculation in helical and direct Tomotherapy plans. Conclusion: The reliability of the tomotherapy TPS in skin dose calculation is maintained by utilizing high spatial resolution for dose computation. The accuracy of TPS validated against the Gafchoromic EBT3 film was within an acceptable gamma-passing rate. 5964 Radiation Biology Efficacy of microwave ablation therapy for uterine fibroids Zhao J. Chen W. Li C. Xiao H. Jin Y. Wang Y. Gao J. Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing, China 1 1 2025 23 1 91 96 03 03 2025 21 01 2025 Background: To investigate the efficacy of laparoscopic combined with microwave ablation therapy in treating multiple uterine fibroids and its impact on patients' quality of life. Materials and Methods: This study included a total of 100 patients with uterine fibroids, divided into a laparoscopic group (50 cases) and a laparoscopic combined with microwave ablation group (50 cases), treated with either laparoscopic surgery alone or laparoscopic combined with microwave ablation therapy. General information of the patients, MRI images before and after treatment, contrast-enhanced ultrasound before and after treatment, intraoperative blood loss, operation time, length of hospital stay, time to postoperative ambulation, VAS score, and ovarian function were collected and analyzed. Results: Compared with laparoscopic surgery alone, the laparoscopic combined with microwave ablation group had lower postoperative pain scores, less intraoperative blood loss, and faster postoperative recovery. There was no significant difference in ovarian function indicators after surgery compared with before surgery, indicating no difference in ovarian function and reserve between the two surgical methods. Additionally, the total effective rate of the combined treatment group reached 98%, significantly higher than the 92% in the laparoscopic group. Imaging analysis showed that the structure of surrounding tissues and vascular layout were restored closer to the normal state after combined treatment. Conclusion: Laparoscopic combined with microwave ablation therapy shows superior efficacy and safety in treating multiple uterine fibroids compared to laparoscopic surgery alone, making it an effective method for treating multiple uterine fibroids, worthy of further clinical promotion and application. 5965 Radiation Biology Investigation of the effects of tissue material on the energy spectrum from Bebig Co-60 High Dose Rate Brachytherapy source based on the Monte Carlo simulation Qomariyah N. Haryanto F. Waris A. Wirawan R. Department of Physics, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Jl. Ganesha 10 Bandung 40132, Indonesia Department of Physics, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Jl. Ganesha 10 Bandung 40132, Indonesia Department of Physics, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Jl. Ganesha 10 Bandung 40132, Indonesia Department of Physics, Faculty of Mathematics and Natural Science, University of Mataram, Jl. Majapahit 62 Mataram 83125, Indonesia 1 1 2025 23 1 97 102 03 03 2025 21 01 2025 Background: High Dose Rate (HDR) brachytherapy is an internal radiation therapy method that delivers high-intensity radiation at >12 Gy/hour dose rate. In clinical practice, the dose calculation is often carried out using the TG-43 However, the method does not consider the effects of patient dimensions and tissue heterogeneity. To overcome this challenge, this study proposed the use of energy spectrum analysis of the source model used. Materials and Methods: GEANT4 Monte Carlo simulation was used to build the geometry of Bebig Co-60 A86 source and radiation interaction system. The interaction physics used Penelope with 1.0 × 108 beam on. The scoring region was spherical with radius 20, 30, 40, and 50 cm as well as material variations of water, muscle, and bone. Results: The spectrum in Compton continuum scattering increased along with a reduction in radius and material density, while the opposite effect was observed at the photoelectric peak. The photoelectric peak ratio experienced 2.96 times increment when the radius of the muscle increased from 20 cm to 30 cm. In addition, the particle count experienced an increment as the radius and medium density of the scoring region increased, with linear regression values of 0.83 for bone and 0.91 for the water and muscle. Conclusion:  Increasing the radius and medium density led to variations in the height of the energy spectrum in Compton continuum and photoelectric regions. These variations caused an 18.42% increase in particle count when transitioning from 20 cm to 30 cm radius in water. 5966 Radiation Biology Study on the relationship between abnormal imaging manifestations of adipose tissue around the primary tumor of colon cancer, genomics and recurrence probability Wang Q. Wang Z. Liu L. Wang H. Department of Basic Medical Sciences, Tianjin Medical College, Tianjin, China Siemens Healthineers, Beijing, China Department of Radiology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, China Department of General Surgery, Tianjin Haihe Hospital, Tianjin, China 1 1 2025 23 1 103 109 03 03 2025 21 01 2025 Background: This study aims to explore the relationship between abnormal imaging manifestations of adipose tissue around the primary tumor of colon cancer, genomics, and recurrence probability, in order to deepen our understanding of the pathological characteristics and molecular mechanisms of colon cancer. Materials and Methods: We collected imaging data of a group of colon cancer patients and obtained relevant genetic information through genomic analysis. Statistical methods were used to quantitatively analyze the abnormal imaging manifestations of adipose tissue around the primary tumor of colon cancer. At the same time, the patient's clinical course is followed to assess the probability of recurrence. Results: The study found that abnormal imaging manifestations of adipose tissue surrounding primary colon cancer tumors were significantly associated with specific gene mutations. Some specific genetic mutations are positively correlated with abnormal signals on imaging, while others are negatively correlated. Furthermore, after long-term follow-up, we observed a relatively higher probability of recurrence in patients with genetic variants associated with imaging abnormalities. Conclusion: The abnormal imaging manifestations of adipose tissue around the primary tumor of colon cancer can be used as one of the auxiliary indicators for molecular classification of colon cancer. The association between specific genetic variants and imaging abnormalities suggests possible molecular mechanisms and provides new clues for personalized treatment. Furthermore, the association of imaging abnormalities with recurrence probability emphasizes its potential importance in prognostic assessment. 5971 Radiation Biology Investigation of the reproducibility of bladder dose- volume histogram (DVH) in prostate tomotherapy using the center of mass of the bladder in daily megavoltage computed tomography (MVCT) images Goli-Ahmadabad F. Mahdavi S.R. Nikoofar A. Zare-Sadeghi A. Vazirinasab H. Bagherzadeh S. Esmaili G. N. Hasani N. Hasani Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Radiation Oncology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Epidemiology and Biostatistics, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran Cellular and Molecular Biology Research Center, Larestan University of Medical Sciences, Larestan, Iran Department of Radiotherapy, Pars Hospital, Tehran, Iran Department of Radiotherapy, Pars Hospital, Tehran, Iran 1 1 2025 23 1 111 120 08 03 2025 21 01 2025 Background: The DVH is the most used radiotherapy formulation. DVH plays a fundamental role in determining dose constraints and side effects. Volume also plays the main role in calculating DVH. In prostate treatment, there is no comprehensive consensus on determining the association between bladder volume (BV) and side effects. Our aim is to investigate the reproducibility of bladder DVH (DVHB). D50%BV (dose received by 50% of BV) is used to analysis DVHB. Materials and Methods: We contoured the bladder of 467 daily MVCT images of fifteen prostate cancer patients who underwent tomotherapy. Using R software 4.2.3, the correlation between the bladder center of mass (XCM, YCM, ZCM), BV with D50%BV were modeled by the mixed model. Two prediction models were presented for D50%BV, the first model was based on BV and (XCM, YCM, ZCM), the second model was based on BV. Results: Statistical analyses revealed that independent factors YCM, ZCM, and BV have a significant influence on the response variable D50%BV. According to mixed model, YCM has a positive correlation with D50%BV, while ZCM or BV has a negative correlation. XCM does not significantly affect D50%BV. Akaike Information Criterion (AIC) index indicated that first model has a higher goodness of fit than second one. Conclusion: Our findings demonstrate that bladder location also affects D50%BV, in addition to BV. It can be concluded that DVHB is not always repeatable as a scientific claim. 5975 Radiation Biology Study on the application of multislice spiral CT in the diagnosis and evaluation of radiotherapy efficacy for esophageal cancer Huang W. Wang L. Ye H. 11st Ward of Tumor Center, Navy Anqing Hospital, Anqing, China Department of Imaging, Affiliated Haimen Hospital of Xinglin College, Nantong University, Nantong, China Department of Radiology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, China 1 1 2025 23 1 121 125 09 03 2025 21 01 2025 Background: To analyze the application value of multislice spiral CT in the diagnosis of esophageal cancer and the evaluation of radiotherapy efficacy. Materials and Methods: A total of 90 esophageal cancer patients treated in our hospital from October 2022 to December 2023 were selected and underwent multislice spiral CT examination. Surgical pathology diagnosis results were used as the gold standard for this study. The diagnostic accuracy of multislice spiral CT for T and N staging of esophageal cancer patients was statistically analyzed. CT perfusion parameters [permeability surface (PS), mean transit time (MTT), blood volume (BV), blood flow (BF)] were compared between the complete remission (CR) group and the non-CR group after radiotherapy. ROC analysis was used to evaluate the predictive efficiency of CT perfusion parameters for CR. Results: The diagnostic accuracy of multislice spiral CT for T1, T2, T3, and T4 staging in esophageal cancer patients was 78.57%, 83.33%, 87.50%, and 95.00%, respectively. The diagnostic accuracy for N0 and N1 staging was 91.89% and 92.45%, respectively. The CR group had lower BV, BF, MTT, and PS compared to the non-CR group (P<0.05). Combined detection of BV, BF, MTT, and PS had a higher sensitivity (95.12%) for predicting CR than single detection methods (66.52%, 76.86%, 72.39%, 77.63%) (P<0.05), with no significant difference in specificity between combined (80.26%) and single detections (78.46%, 81.24%, 82.71%, 80.89%) (P>0.05). Conclusion: Multislice spiral CT can accurately diagnose T and N staging in esophageal cancer patients. Combined detection of CT perfusion parameters BV, BF, MTT, and PS can improve the sensitivity of predicting CR, providing important reference value. 5974 Radiation Biology Comparison between sagittal sequences in magnetic resonance imaging of the lumbar spine with fat saturation and different phase encoding directions Papaioannou M. Tsikrika A. Roka V. Lavda P. Pantazis N. Sakkas G.K. Dardiotis E. Bakas A. Mavroidis P. Lavdas E. Department of Medical Imaging, Animus Kyanoys Stavros, Larissa, Greece Department of Radiology, University Hospital of Larissa, Larissa, Greece Health Center of Farkadona, Trikala, Greece Department of Biomedical Sciences, University of West Attica, Athens, Greece Department of Radiology, Dimokritos, Alexandroupoli, Greece University of Thessaly, Department of Physical Education and Sports Science, Trikala, Greece Department of Neurology, University Hospital of Larissa, Larissa, Greece Department of Biomedical Sciences, University of West Attica, Athens, Greece Department of Radiation Oncology, University of North Carolina, Chapel Hill, NC, USA Department of Medical Imaging, Animus Kyanoys Stavros, Larissa, Greece 1 1 2025 23 1 127 133 09 03 2025 21 01 2025 Background: Comparison of three different sagittal sequences with fat suppression in the lumbar spine magnetic resonance imaging (MRI), evaluating several image characteristics and changing the phase direction. Materials and Methods: Forty-five subjects (20 males, 25 females, mean age 50 years old) participated in this retrospective study in an MRI machine of 1.5 Tesla (GE Signa Hdx). We compared three fat-saturated sequences {T2 Weighted (T2W) Fast Spin Echo (FSE) Fat Saturation (FS) with phase direction superior-inferior (S/I), T2W Short Tau Inversion Recovery (STIR) with phase direction superior-inferior (S/I) and T2W STIR with phase direction (A/P)}. A qualitative analysis was performed, while two experienced radiologists evaluated the images. The statistical analysis was determined by Kruskal–Wallis non-parametric test. Results: The T2W FSE FS was superior in almost all studied parameters {total image quality, presence of artifacts, artifacts in 4th lumbar vertebra (L4) - 1st Sacral vertebra (S1), depiction of lesions on vertebral bodies, depiction of lesions on L4-S1 region, sharpness} in comparison with T2W STIR sequences with statistically significant difference (p<0.001). The STIR sequences exceeded the T2W FSE FS in the fat saturation effectiveness with a statistically significant difference (p<0.001). Conclusion: The T2 Weighted (T2W) Fast Spin Echo (FSE) Fat Saturation (FS) was superior in the depiction of pathology and normal anatomy, eliminating many artifacts in comparison with T2 Short Tau Inversion Recovery (STIR) sequences, especially in the L4 vertebra– S1 vertebra anatomic region, between three under-study sequences. Choosing the appropriate sagittal fat-saturated sequence in each clinical question is useful to avoid misdiagnosis due to technical artifacts.  5984 Radiation Biology Feasibility study of an automatic injection plan for coronary computed tomography angiography using 80 kilovolt and very low iodine dose Yang Y. Ge S. Chen Y. Jing C.P. Department of Radiology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian 223300, China Department of Radiology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian 223300, China Department of Radiology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian 223300, China Department of Radiology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian 223300, China 1 1 2025 23 1 135 140 11 03 2025 21 01 2025 Background: To explore the feasibility of using various ultra-low contrast flow rates on the coronary CTA (CCTA) when the tube voltage is set at 80KV automatically on 3rd-generation dual-source CT. Materials and Methods: A total of 180 patients with suspected coronary atherosclerotic disease were randomly divided into two groups, each subdivided into seven BMI-based subgroups (≤ 25, 25-26, 26-27, 27-28, 28-29, 29-30, > 30). The experimental group (A) used individualized injection rates (2.8-3.4 ml/s) with 350 mg I/ml contrast agent based on BMI, with a fixed injection time of 10s. The control group (B) used a fixed injection rate of 4.0 ml/s and a fixed injection time of 12s. Both groups employed prospective ECG-gated scanning. Image quality, effective radiation dose, and contrast agent dosage were compared using the Student's t-test. Results: In Group B, the coronary artery CT value exceeded the optimal diagnostic range (300-450 HU) recommended by experts. Group A had significantly lower CT values, SNR, and CNR compared to Group B (P < 0.05), but the enhancement in Group A was closer to the optimal diagnostic range. There was no significant difference in subjective image scores between the groups (P > 0.05), with consistent scoring between two directors (ICC: 0.612-0.852). Both groups had similar effective doses (P > 0.05). The contrast agent dosage in Group A was significantly lower than in Group B (P < 0.05). Conclusion: The optimized contrast injection scheme can significantly reduce the amount of contrast agent and obtain better image quality.   Background: To explore the feasibility of using various ultra-low contrast flow rates on the coronary CTA (CCTA) when the tube voltage is set at 80KV automatically on 3rd-generation dual-source CT. Materials and Methods: A total of 180 patients with suspected coronary atherosclerotic disease were randomly divided into two groups, each subdivided into seven BMI-based subgroups (≤ 25, 25-26, 26-27, 27-28, 28-29, 29-30, > 30). The experimental group (A) used individualized injection rates (2.8-3.4 ml/s) with 350 mg I/ml contrast agent based on BMI, with a fixed injection time of 10s. The control group (B) used a fixed injection rate of 4.0 ml/s and a fixed injection time of 12s. Both groups employed prospective ECG-gated scanning. Image quality, effective radiation dose, and contrast agent dosage were compared using the Student's t-test. Results: In Group B, the coronary artery CT value exceeded the optimal diagnostic range (300-450 HU) recommended by experts. Group A had significantly lower CT values, SNR, and CNR compared to Group B (P < 0.05), but the enhancement in Group A was closer to the optimal diagnostic range. There was no significant difference in subjective image scores between the groups (P > 0.05), with consistent scoring between two directors (ICC: 0.612-0.852). Both groups had similar effective doses (P > 0.05). The contrast agent dosage in Group A was significantly lower than in Group B (P < 0.05). Conclusion: The optimized contrast injection scheme can significantly reduce the amount of contrast agent and obtain better image quality. 5987 Radiation Biology Estimation of peak skin dose in cardiac interventional procedure using radiation dose structured report-an indirect method Nair S.S. Gupta S.K. Shine N.S. Thomas K.T. George V. Thomas P. Gopakumar M.K. Nair S.S. Department of Physical Science, Banasthali Vidyapith, Jaipur, India Department of Physical Science, Banasthali Vidyapith, Jaipur, India Department of Physical Science, Banasthali Vidyapith, Jaipur, India Department of Radiation & Oncology, General Hospital Ernakulam, India Department of Cardiology, General Hospital Ernakulam, India Department of Cardiology, General Hospital Ernakulam, India Department of Cardiology, General Hospital Ernakulam, India Department of Radiotherapy and Oncology, Manipal, India 1 1 2025 23 1 141 146 12 03 2025 21 01 2025 Background: The purpose of this study is to estimate PSD indirectly using a Radiation Dose Structured Report (RDSR) from Cath lab interventional procedures. The estimated dose was then compared with direct measurements using films. Materials and Methods: Information on radiation exposure and dosage associated with a specific interventional radiology procedure is provided in the RDSR document. The RDSR produced by the machine was verified using slab phantom, Gafchromic XRV3 film, and Ray Safe X2 detector. The PSD is estimated by adding the intensely projected beam dose in acquisition mode with the fluoroscopic dose percentage obtained from the RDSR. During the procedure, Gafchromic films were used to measure PSD directly. Then, the estimated PSD was compared with the measured PSD. Result: The PSDmes and PSDcal in this study showed an average difference of 0.12 Gy (8%). The Wilcoxon Signed-Rank test has a p-value of 0.08 and the Spearman's rank correlation coefficient (rs) of 0.97 indicates a very strong positive correlation between the two variables. Conclusion: The Statistical analysis shows that the estimation of PSD using RDSR is reliable for monitoring the patient. This method may help the cardiologist to follow up of the patients to give extra care to skin reactions. A safe standard work practice will certainly monitor the prevention of undesirable consequences of radiation. 5998 Radiation Biology The Aurora-A/NPAT/Wnt-β-catenin pathway enhances the metastasis and stemness of lung cancer cells Zhao Y. Zhao Y. Li P. Fu X. Duan J. Tang Y. Ma Y. Zhou Q. Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China Department of Medical Oncology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, China 1 1 2025 23 1 147 154 15 03 2025 21 01 2025 Background: Aurora-A kinase (Aurora-A) serves as an oncogene in many cancers, but whether its expression is linked to the stemness of lung cancer (LC) cells remains obscure. Hence, this study probed the impact as well as mechanism of Aurora-A in affecting the stemness of LC cells. Materials and Methods: Functional assays were implemented to assess the malignant behaviors along with stemness of LC cells. Western blot analysis detected the stemness markers together with the Wnt-β-catenin pathway-linked genes protein levels. TOP/FOP-Flash reporter assay assessed the activity of the Wnt/β-catenin pathway. Hematoxylin and eosin staining as well as immunohistochemistry analysis of tumor tissues were implemented. Results: Our findings indicated that Aurora-A was up-regulated in LC cells, and silenced Aurora-A hindered LC cell proliferation, migration, invasion along with stemness. Nuclear protein, coactivator of histone transcription (NPAT) was also high-expressed in LC cells, and was positively modulated by Aurora-A. Silenced NPAT inhibited LC cell malignant behaviors along with stemness. Aurora-A activated the Wnt/β-catenin pathway and promoted LC cell malignant behaviors along with stemness via regulating NPAT. In an established xenograft model, Aurora-A inhibition reduced tumor growth, metastasis along with stemness in vivo. Conclusion: In summary, Aurora-A/NPAT/Wnt-β-catenin signaling pathway accelerates LC cell malignant behaviors along with stemness. The modulation of Aurora-A might be an underlying therapeutic approach in LC patients. 5999 Radiation Biology Application of CT/MRI Fusion Imaging in the Diagnosis and Staging of Liver Cancer: A Comparative Study Teng F. Liu C. Feng Z. Li X. Department of Radiology, The Second Norman Bethune Hospital of Jilin University, Changchun, 130000, China Department of Radiology, The Second Norman Bethune Hospital of Jilin University, Changchun, 130000, China Department of Radiology, The Second Norman Bethune Hospital of Jilin University, Changchun, 130000, China Department of Radiology, The Second Norman Bethune Hospital of Jilin University, Changchun, 130000, China 1 1 2025 23 1 155 161 15 03 2025 21 01 2025 Background: The purpose of this study is to evaluate the value of computed tomography (CT) / magnetic resonance imaging (MRI) fusion imaging technology in the diagnosis and staging of liver cancer. Materials and Methods: Sixty patients with primary hepatocellular carcinoma diagnosed by histopathology and cytology who visited our hospital from April 2021 to May 2023 were selected. All patients underwent CT and MRI single mode scans, as well as CT/MRI fusion imaging scans. Based on the pathological diagnosis results, the consistency and accuracy of three imaging methods in evaluating the number, maximum diameter, and staging of liver cancer patients were compared. Results: CT/MRI fusion imaging can more accurately determine the number (F=8.62, P<0.001), maximum diameter (F=86.69, P<0.001), and pathological staging of liver cancer lesions compared to single CT or MRI. In terms of determining the number of tumors, CT/MRI fusion imaging is superior to CT (Kappa 0.695 vs 0.654) and MRI (Kappa 0.872 vs 0.695). In terms of evaluating the maximum pathway, CT/MRI fusion imaging has the highest consistency with MRI results (Kappa 0.931), which is significantly better than CT (Kappa=0.818). In staging judgment, CT/MRI fusion imaging improves the diagnostic accuracy of stages II and III tumors.  Conclusion: The CT/MRI fusion imaging technology, which integrates the advantages of CT and MRI imaging examination modes, can improve the display rate, quantitative and staging evaluation accuracy of liver cancer lesions. And it is worthy of further promotion to improve the imaging fine diagnosis level of liver cancer. 6000 Radiation Biology Efficacy and safety of hypofractionated radiotherapy and conventional fractionated radiotherapy in the treatment of early breast cancer patients after breast-conserving surgery Tang J. Xu X. Chen M. Liu L. Zhan Z. Department of Oncology, Pingxiang People’s Hospital, Pingxiang, Jiangxi 337055, China Department of Oncology, Pingxiang People’s Hospital, Pingxiang, Jiangxi 337055, China Department of Oncology, Pingxiang People’s Hospital, Pingxiang, Jiangxi 337055, China Department of Oncology, Pingxiang People’s Hospital, Pingxiang, Jiangxi 337055, China Department of Oncology, Pingxiang People’s Hospital, Pingxiang, Jiangxi 337055, China 1 1 2025 23 1 163 168 15 03 2025 21 01 2025 Background: To compare the efficacy as well as safety of hypofractionated radiotherapy (HFRT) with conventional fractionated radiotherapy (CFRT) for the treatment of early breast cancer (EBC) patients after breast-conserving surgery (BCS). Materials and Methods: Clinical data of 126 early breast cancer patients diagnosed and treated in our hospital from March 2021 to June 2023 were retrospectively analyzed. Patients were divided into HFRT group and CFRT group. The CFRT group accepted conventional segment radiotherapy. The HFRT group accepted hypofractionated radiotherapy. The clinical effect, breast beauty effect, incidence of adverse reactions, duration of hospital stay, local recurrence as well as distant metastasis in two groups were compared. Results: The HFRT and CFRT group showed no statistical difference in total response rate, breast beauty effect, length of hospital stay, local recurrence and distant metastasis rate (P>0.05). The incidence of bone marrow suppression, acute skin adverse reactions and radiation pneumonia in the HFRT group was lower than that in the CFRT group (P<0.05). The advanced skin adverse reactions were not statistically different between groups (P>0.05). Conclusion: Conventional radiotherapy and hypofractionated radiotherapy have similar clinical efficacy and safety for early breast cancer patients after BCS, while hypofractionated radiotherapy with the advantages of less radiotherapy times, short course of treatment and higher safety is valuable for clinical treatment. 6001 Radiation Biology Computed tomography manifestations and clinical features of acquired immune deficiency syndrome patients with cervical lymph node tuberculosis Wang H. Dai X. Liu X. Li C. Shu W. Department of Medical Imaging, Chongqing Public Health Medical Treatment Center, Chongqing 400036, China Department of Medical Imaging, Chongqing Public Health Medical Treatment Center, Chongqing 400036, China Department of Medical Imaging, Chongqing Public Health Medical Treatment Center, Chongqing 400036, China Department of Medical Imaging, Chongqing Public Health Medical Treatment Center, Chongqing 400036, China Department of Medical Imaging, Chongqing Public Health Medical Treatment Center, Chongqing 400036, China 1 1 2025 23 1 169 173 15 03 2025 21 01 2025 Background: To analyze the computed tomography (CT) findings and clinical features of acquired immune deficiency syndrome (AIDS) patients with cervical lymph node tuberculosis (CLNT). Materials and Methods: The clinical data of 100 patients diagnosed with CLNT in our hospital from January 2020 to December 2022 were retrospectively analyzed. Based on whether AIDS was combined, 20 cases of CLNT patients with AIDS were included in observation group (OG), and 80 cases of CLNT patients without AIDS were included in control group (CG). CT imaging features and general clinical data were analyzed and compared between groups. Results: Cervical lymph nodes in the OG showed irregular shape, blurred boundary, and short diameter >3 cm, with statistical difference relative to the CG (P<0.05). The complete necrosis type of cervical lymph node necrosis in the OG presented higher relative to the CG (P<0.05). The OG was more likely to involve lymph nodes in region I and II of the neck (P<0.05). Besides, the lymph node lesions involving ≥3 regions and ≥4 regions, proportion of males and mean age of patients were elevated in the OG (P<0.05). The mean CD4+T lymphocyte count was lower in the OG (P<0.05). The OG also showed higher incidence of clinical symptoms and the proportion of combined clinical symptoms ≥3 relative to the CG (P<0.05). Conclusion: The CT manifestations of AIDS patients have certain commonality compared with non-AIDS patients, which might provide clues for clinical diagnosis. 6002 Radiation Biology MicroRNA-18a-5p targets Sec61 translocon alpha 1 subunit to repress hepatocellular carcinoma cell growth before chromradiotherapy Chen Q. Wang M.M. Meng X-K. Wu Q.L. Department of Infectious Diseases, Ningbo Yinzhou No.2 Hospital, Yinzhou City, Ningbo, China Department of Clinical Laboratory, The Second People’s Hospital of Nantong, Nantong City, Jiangsu, China Department of Digestive Surgery Ward 2, Xi'an International Medical Center Hospital, Xi'an City, Shaanxi, China Department of Digestive Surgery Ward 2, Xi'an International Medical Center Hospital, Xi'an City, Shaanxi, China 1 1 2025 23 1 175 183 15 03 2025 21 01 2025 Background: Hepatocellular carcinoma (HCC) cell development was investigated in relation to the regulation of Sec61 translocon alpha 1 subunit (SEC61A1) by microRNA (miR)-18a-5p. Materials and Methods: After collection of clinical samples, the transfection of interfering vectors of miR-18a-5p or SEC61A1 was into HCC cells to figure out their roles in development of HCC. The Pearson test and starBase analyzed the association and target prediction of miR-18a-5p. Subsequently, through the dual luciferase reporter experiment, SEC61A1 can be regulated via miR-18a-5p. The salvage experiment revealed that miR-18a-5p influence the degradation process of Hepatocellular carcinoma through combining SEC61A1. Results: In HCC cells, we found that SEC61A1 was elevated and miR-18a-5p was downregulated. HCC cells deteriorating was considerably slowed down by the enhanced miR-18a-5p level. The outcomes demonstrated that miR-18a-5p can interact and regulate SEC61A1. Additionally, the effects of earlier therapy on HCC cell proliferation can be restored by overexpressing SEC61A1. Conclusion: Overall, in HCC cells and tissues miR-18a-5p was significantly downregulated, and inhibited the proliferative ability of HCC cells by targeting SEC61A1. 6003 Radiation Biology Meta-analysis of curative effect of minimally invasive radical hysterectomy and open radical hysterectomy with primary chemo-radiation in patients with cervical cancer Pan J. Hang H. Hu Q. Department of Gynecology, Huzhou Maternity & Child Health Care Hospital, Zhejiang, China Department of Gynecology, Huzhou Maternity & Child Health Care Hospital, Zhejiang, China Department of Gynecology, Huzhou Maternity & Child Health Care Hospital, Zhejiang, China 1 1 2025 23 1 185 191 15 03 2025 21 01 2025 Background: The comparative analysis of minimally invasive radical hysterectomy (MIRH) and open radical hysterectomy (ORH) in cervical cancer (CC) patients undergoing primary chemoradiation has yielded divergent and contentious study conclusions. Materials and Methods: A comprehensive literature search was conducted across PubMed, Web of Science core database, Nature, Science Direct, and others, covering the period from January 2007 to the present. Results: Ten relevant articles were identified, encompassing 4,148 CC patients who underwent radical hysterectomy, with 1,949 (47.0%) undergoing MIRH and 2,199 (53.0%) undergoing ORH. Among these patients, 767 experienced recurrence, and 223 succumbed to the disease. The analysis revealed a slightly higher recurrence rate in patients who underwent MIRH versus ORH [OR=1.25, 95% CI=1.02~1.54, Z=2.16, P=0.03], demonstrating statistical significance (P<0.05). However, the mortality rate in MIRH patients was marginally superior to in ORH patients [OR=1.02, 95% CI=0.76 ~ 1.37, Z=0.11, P=0.91] (P>0.05). MIRH was associated with a slightly lower complication rate versus ORH [OR=0.40, 95% CI=0.28 ~ 0.57, Z=5.02, P<0.00001], and a significantly reduced incidence of perioperative blood transfusions [OR=0.19, 95% CI=0.05 ~ 0.58, Z=4.18, P<0.0001]. Conclusion: The findings suggest that ORH is linked to lower recurrence and mortality rates versus minimally invasive surgery, establishing its potential advantage in the treatment of CC. 6004 Radiation Biology Comparison of pediatric doses of cone beam computed tomography and panoramic radiography in three age groups Eren H. Deniz Y. Ata G.C. Sessiz R. Department of Dental and Maxillofacial Radiology, Dentistry Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye Department of Dental and Maxillofacial Radiology, Dentistry Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye Department of Dental and Maxillofacial Radiology, Dentistry Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye Department of Dental and Maxillofacial Radiology, Dentistry Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye 1 1 2025 23 1 193 199 15 03 2025 21 01 2025 Background: A wide variety of radiation dose values can occur in Cone Beam Computed Tomography (CBCT) devices with different field of view (FOV) sizes. Radiation dose of current CBCT devices have been considerably reduced. This study compared effective radiation doses and organ absorption doses obtained from panoramic and CBCT imaging at various FOV sizes in children aged five, ten, and fifteen years. Materials and Methods: To calculate the organ doses and effective doses, a dose calculation software PC-based Monte Carlo (PCXMC) 2.0 based on Monte-Carlo simulation was used for CBCT and panoramic exposures. Both absorbed and effective doses were calculated for the simulated phantoms of 5, 10 and 15 years old separately. Results: The organ doses of thyroid and salivary glands measured with 6*6 ECO Scan CBCT were found to be lower when compared with panoramic radiography organ doses. Panoramic radiography effective doses were lower than all other CBCT modes in all age groups. Conclusion: Although it is stated that this study does not have diagnostic data, it is thought that 6×6 ECO Scan Mode of Newtom CBCT can be chosen instead of panoramic radiography in children aged 5 years due to the dose differences. 6055 Radiation Biology High-intensity focused ultrasound inhibits lncRNA MAFA-AS1-modulated stability of HIF-1 mRNA in an m6A-dependent manner to suppress aerobic glycolysis and adriamycin resistance in ovarian cancer Huang M. Peng Y. Sun Y. Gao W. Fei J. Zhou C. Xie L. Zhou L. Wu W. Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China Department of Ultrasound, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, China 1 1 2025 23 1 201 210 05 04 2025 21 01 2025 Background: High-intensity focused ultrasound (HIFU) represents a therapeutic medical procedure that operates by inducing ablation and mechanical disruption. Despite its established efficacy, its potential impact on tumor chemotherapy remains uncertain. Long noncoding RNA (lncRNA) MAFA-AS1 facilitated cancer cell proliferation and fostering drug resistance, but the precise significance and functional implications of MAFA-AS1 in the context of ovarian cancer (OC) remain largely unexplored. The objective of this experiment is to further investigate the potential of HIFU in inhibiting the chemotherapy resistance mechanism of OC. Materials and Methods: Five types of human ovarian cancer cells were employed in this study. There were four different groups, namely MAFA-AS1 siRNA, si-NC, pcDNA3.1-HIF-1 (hypoxia-inducible factor-1), and pcDNA3.1-control. Quantitative real-time PCR, cell proliferation assay, apoptosis assay, western blot assay, subcellular fractionation, aerobic glycolysis analysis, RNA immunoprecipitation (RIP), and luciferase reporter assay were conducted for experimentation and validation. Results: Significant upregulation of MAFA-AS1 in OC cells was observed. Through loss-of-function experiments based on HIFU, we unraveled its oncogenic functions in OC. MAFA-AS1 was discovered to bind to HIF-1 mRNA, thereby enhancing its expressed stability. Further investigations revealed an interaction between MAFA-AS1 and fat mass and obesity-associated protein (FTO), positively modulating HIF-1 mRNA stability in an FTO-dependent manner. Importantly, MAFA-AS1 exerts its effects on OC by acting through HIF-1. Conclusion: The study underscores the role of MAFA-AS1 in promoting aerobic glycolysis and chemical resistance in OC by up-regulating HIF-1 expression, suggesting that targeting MAFA-AS1 holds promise as a therapeutic strategy for OC patients undergoing chemotherapy. 6056 Radiation Biology Specific inhibitors of PI3K, mTOR and AR exhibit strong synergism and radiosensitization in human prostate cell lines Maleka S. Serafin A.M. Akudugu J.M. Department of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa Department of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa Department of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa 1 1 2025 23 1 211 223 05 04 2025 21 01 2025 Background: Single agent use of chemotherapy in prostate cancer is very limiting, as large doses are often required for tumour control and can lead to elevated systemic toxicity. Targeting of survival proteins of the epidermal growth factor receptor (EGFR), phosphoinositide 3-kinases (PI3K), mammalian target of rapamycin (mTOR) and androgen receptor (AR) pathways with cocktails of specific inhibitors might yield optimum therapeutic benefit with minimal toxicity. Materials & Methods: The modes of interaction of the dual inhibitor of PI3K and mTOR (NVP-BEZ235), EGFR inhibitor (AG-1478), and AR inhibitor (MDV3100) in in vitro cultures of four human prostate cell lines (DU145, LNCaP, BPH-1 and 1542N) were evaluated as cocktails, using clonogenic cell survival, subsequent to validation of the androgen dependency. Components of cocktails (Cocktail 1 (AG-1478 and NVP-BEZ235), Cocktail 2 (NVP-BEZ235 and MDV3100), and Cocktail 3 (MDV3100 and AG-1478)) were used at equivalent concentrations for 50% cell killing. Combination indices (CI) for the cocktails were determined and used as descriptors of inhibitor interaction. Radiomodulatory effects of inhibitor cocktails were also evaluated. Results: Inhibitor cocktails selectively showed strong to very strong synergism and radiosensitization. Concurrent inhibition of PI3K/mTOR and AR could potentially be of better therapeutic benefit than inhibition of EGFR and PI3K/mTOR or AR and EGFR, as the potential benefit of EGFR targeting was found to be limited. Conclusion: These data may guide the design of potent treatment approaches for prostate cancer. 6057 Radiation Biology Evaluation of Monaco dose calculation errors for out-of-field regions in intensity modulated radiotherapy of nasopharyngeal cancer Sardari D. Saeedzadeh E. Karimkhani L. Mahdavi S.R. Department of Radiation Medical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Radiation Medical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Radiation Medical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran Radiation Biology Research Center, Medical Physics Department, Iran University of Medical Sciences, Tehran, Iran 1 1 2025 23 1 225 232 05 04 2025 21 01 2025 Background: The aim of this study is to assess the accuracy of dose calculations by Monaco Treatment Planning System (TPS) for critical organs positioned in out-of-field (OOF) regions during two intensity-modulated radiation therapy (IMRT) techniques in patients diagnosed with nasopharyngeal cancer (NPC). Materials and Methods: Computed tomography (CT) images from 10 NPC patients (aged 54-77 years) were used for treatment planning using 7 and 11 fields IMRT techniques with the Monaco TPS. Doses for organs at risk (OARs) in OOF regions, including the eyes, lenses, and optic nerves, were calculated using the TPS and compared with measurements obtained from the OCTAVIUS 4D phantom. Additionally, dose distributions derived from TPS calculations were compared with measurements using the gamma analysis method, with a threshold dose set at 10% of the maximum dose. Results: Although gamma pass rates exceeded 95% for all patients when OOF regions were excluded, measurements indicated that the Monaco TPS generally underestimated doses to OOF organs by approximately 25%. This underestimation tended to increase with lower dose values, and TPS errors varied across different tissues, including the eyes, lenses, and optic nerves. Conclusion: In conclusion, the Monaco TPS demonstrates significant underestimation errors in dose calculations, especially for organs located in OOF regions during IMRT for NPC patients. Considering the potential risk of secondary cancers, it is imperative to prioritize meticulous attention to ensure precise dose estimation in OOF regions by the TPS. 6062 Radiation Biology A meta-analysis examining the risk factors associated with pneumothorax during CT-guided percutaneous lung biopsy Xiaoxing J. Dongnv W. Yitian W. Ling M. Wenbin J. Shuzong Y. Department of Radiology, Medical Community of Linhai Second People's Hospital, Linhai, China Department of Radiology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China Department of Radiology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China Key Laboratory of evidence-based Radiology of Taizhou, Linhai, Zhejiang, China Department of Radiology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China Department of Radiology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China 1 1 2025 23 1 233 238 06 04 2025 21 01 2025 Background: The study aimed to systematically evaluate the risk factors for pneumothorax after computed tomography (CT)-guided percutaneous lung biopsy. Materials and Methods: We conducted a search of several databases, including PubMed, Embase, the Cochrane Library, Web of Science, and CNKI, until May 2021, focusing on research related to risk factors for pneumothorax following CT-guided percutaneous lung biopsy. After screening and extracting the data, we conducted a meta-analysis using RevMan software (version 5.3) to calculate the odds ratio (OR) and 95% confidence interval (CI) for each relevant risk factor. Statistical significance was set at P<0.05. Results: Our study included 28 articles and 16,667 patients who underwent CT-guided lung biopsy, of whom 4,566 patients developed pneumothorax (27.40%). Our results showed that the incidence of pneumothorax was lower in the group with pulmonary nodules ≥ 2 cm, and the difference was statistically significant (OR=0.61, 95% CI=0.43–0.86, P=0.004). Additionally, patients with emphysema had a higher risk of developing a pneumothorax after the procedure, and the difference was statistically significant (OR=2.5, 95% CI=1.62–3.87, P<0.0001). Correlation analysis of sex, lung nodule size, needle track length, and CT-guided lung nodule biopsy showed no significant differences (P>0.05). Conclusion: Our findings indicate that pulmonary nodules smaller than 2 cm and emphysema significantly increase the risk of pneumothorax after puncture. Clinicians should be vigilant in monitoring patients with these risk factors after the procedure. 6067 Radiation Biology Predicting prostate cancer radiotherapy complications: An integrated approach using radiomics, dosiomics, and machine learning Sadati E. Hashemi B. Mahdavi S.R. Nikoufar A. Abdollahi H. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Medical Physics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Radiation Oncology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Integrative Oncology, BC Cancer Research Institute, Vancouver, BC, Canada 1 1 2025 23 1 239 244 07 04 2025 21 01 2025 Background: We aimed to develop a robust prognostic model for assessing the risk of complications associated with radiotherapy in prostate cancer patients using radiomics and dosiomics feature and machine learning. Materials and Methods: A cohort of 60 patients undergoing pelvic radiation therapy was analyzed. The patients’ radiomics and dosiomics features were extracted from segmented bladder and rectum regions in CT images, as well as 3D dose distribution data, respectively. Classifier algorithms, such as eXtreme Gradient Boosting (XGBoost), Decision Tree (DT), Support Vector Machines (SVM), K-Nearest Neighbor (KNN), Logistic Regression (LR), Random Forest (RF), and Multilayer Perceptron (MLP) were used for prediction modeling. A 5-fold cross-validation method was used to evaluate the predictive classification of patients with and without proctitis and cystitis. The area under the receiver operating characteristic curve (AUC) was used for comparing models’ performance, as well assessing their specificity and accuracy metrics. Results: Various combinations of feature selection and classifier algorithms evaluated on both training and test datasets revealed that for bladder toxicity, the Relief+KNN dosiomics model, Boruta+SVM radiomics model, and the combined radiomics and dosiomics model with ANOVA+XGBoost show the highest AUCs of 0.76, 0.68, and 0.67, respectively. Regarding the rectal toxicity, the best-performing models were Boruta+KNN for dosiomics (AUC 0.83), ANOVA+RF for radiomics (AUC 0.72), and ANOVA+XGBoost for the combined radiomics and dosiomics (AUC 0.71). Conclusion: Our study demonstrated the effectiveness of diverse algorithms leveraging quantitative features extracted from CT imaging and 3D dose distribution data in predicting post-radiotherapy complications in prostate cancer patients. 6110 Radiation Biology Value of color Doppler ultrasonography in diagnosis of carotid atherosclerotic plaque in cerebral infarction patients Guo L. Guo X. Kong R. Department of Neurology, Yantaishan Hospital, Yantai City, Yantai, Shandong, China Department of Gerontology, Yantaishan Hospital, Yantai City, Yantai, Shandong, China Department of Functional Neurology, Wuxi People's Hospital, Wuxi, Jiangsu, China 1 1 2025 23 1 245 248 21 04 2025 21 01 2025 Background: To assess the value of color Doppler ultrasonography (CDUS) in diagnosing carotid atherosclerotic plaque (CAP) in cerebral infarction (CI) patients. Materials and Methods: Totally 100 CI patients in our hospital from March 2021 to March 2023 were included in the study group (SG), and another 98 patients who received physical examination at the same time were included in the control group (CG). CDUS was performed to determine the type, distribution and total detection rate of carotid atherosclerotic plaque, and to evaluate carotid blood flow parameters, carotid intima-media thickness (IMT) and stenosis degree in the two groups. Results: No significant difference of plaque in the proportion of external carotid artery (ECA), internal carotid artery (ICA), common carotid artery (CCA) and carotid artery bifurcation between the two groups (P>0.05). Relative to the CG, the detection rate of each type of carotid atherosclerotic plaque in the SG was significantly higher (P=0.03 and P<0.001), peak systolic flow velocity (PSV), minimum diastolic flow velocity (EDV), and resistance index (RI) of carotid blood flow were lower, and IMT was higher in the SG (P<0.05), and the proportion of mild, moderate and severe carotid artery stenosis in the SG were higher (P<0.01 and P<0.001). Conclusion: CDUS can detect the distribution and quantity of plaques, determine the type and stability of plaques, and assess the carotid blood flow parameters and stenosis, which is valuable for the diagnosis of CAP in CI, and guides the formulation of clinical treatment plan. 6111 Radiation Biology Evaluation of imaging diagnostics in dizziness induced by cerebral small vessel disease: A focus on transcranial Doppler and CT Wei Y. Wang Q. Yang J. Department of Neurology, Shanghai Construction Engineering Hospital, Shanghai, China Department of Neurology, Shanghai Construction Engineering Hospital, Shanghai, China Department of Neurology, Shanghai Construction Engineering Hospital, Shanghai, China 1 1 2025 23 1 249 254 21 04 2025 21 01 2025 Background: This study aims to evaluate the therapeutic efficacy of hyperbaric oxygen (HBO) as adjuvant therapy for patients with cerebral small vessel disease (CSVD) related dizziness and to assess the impact of this treatment on diagnostic indicators from both transcranial Doppler (TCD) and computed tomography (CT). Materials and Methods: A prospective randomized controlled study was conducted from January 2019 to December 2023, including 200 patients with CSVD related dizziness. They were randomly assigned to either the HBO group or the control group. The control group received betahistine mesylate tablets, while the HBO group received HBO in addition to the control group treatment. Various parameters including Dizziness Handicap Inventory (DHI) scores, cerebral artery blood flow velocity (Vm), resistance index (RI), pulsatility index (PI), cystatin (Cys), homocysteine (HCY), hypersensitive c-reactive protein (hs-CRP) levels, and CT imaging for grading cerebral white matter degeneration were assessed before and after treatment. Results: After treatment, DHI scores in both groups decreased significantly, with the HBO group showing greater improvement compared to the control group. Hemodynamic parameters (Vm, RI, PI) improved significantly in both groups, with greater improvements seen in the HBO group. White matter lesion grading and levels of Cys, HCY, and hs-CRP were significantly lower in the HBO group post-treatment compared to the control group. Conclusions: HBO as adjuvant therapy demonstrates significant therapeutic benefits for CSVD related dizziness. It effectively alleviates symptoms, improves hemodynamic parameters, enhances white matter lesion assessment outcomes, and promotes neurological function recovery. 6112 Radiation Biology 3D FIESTA MRI for trigeminal neuralgia diagnostic tools : A case report Fatmawati H. Adji N.K. Bumi C. Department of Radiology, Faculty of Medicine, University of Jember, Indonesia Department of Neurosurgery, Faculty of Medicine, Univeof Jember, Indonesiarsity Department of Epidemiology, Faculty of Public Health, University of Jember, Indonesia 1 1 2025 23 1 255 258 21 04 2025 21 01 2025 Background: Trigeminal neuralgia (TN) is a severe condition presented with abrupt pain in the area innervated by one or more branches of the fifth cranial nerve. The pain is usually described as sudden, unilateral, severe, brief, stabbing, and recurrent sensation. Case Description: A 60-year-old female with a left-sided facial has had pain for two years.  She was referred by a neurosurgeon diagnosed as having left stabbing pain that radiated down the distribution of the third-division trigeminal nerve. An MRI 1.5T with conventional and high-resolution protocols used the sequences three-dimensional magnetic resonance imaging (3D MRI) study included fast imaging employing steady-state acquisition. According to the ability of 3D-FIESTA MRI, it is used to demonstrate the anatomy of the trigeminal complex. The 3D FIESTA sequence has the ability to demonstrate the compression and displacement of the nerve. Conclusion: 3D FIESTA MRI is a useful tool to assess the trigeminal system and may describe the recent understanding pathology of TN. 6113 Radiation Biology Diagnosis of fetal transverse facial cleft by magnetic resonance imaging negative interval scanning sequence: a case report Chang L. Shi L. L. Chang1 and L. Shi2 1Department of Radiology, Tangshan Gongren Hospital, Tangshan, 063000, China 2Department of Radiology, Tangshan Maternal and Child Health Hospital, Tangshan, 063000, China Department of Radiology, Tangshan Maternal and Child Health Hospital, Tangshan, 063000, China 1 1 2025 23 1 259 262 21 04 2025 21 01 2025 The Transverse facial cleft is a rare congenital malformation of the fetal face. Color Doppler ultrasound is one the common methods of screening for this condition, but its diagnostic accuracy can be affected by the position of the placenta, the shape of the pregnant woman’s body, and whether the fetus is oriented in an open or closed position. Magnetic resonance imaging has the advantages of high soft tissue resolution and wide field of vision, which facilitate objective and comprehensive evaluation of fetal development. It can be an important supplementary means of prenatal screening. We here report a case of fetal bilateral lip angle asymmetry revealed by color Doppler ultrasound screening at 26 weeks’ gestation. Magnetic resonance image negative interval scanning was used to diagnose transverse facial cleft, which was confirmed after induced labor. We also retrospectively analyzed the process of embryonic development, imaging manifestations, and treatment methods of transverse facial cleft.