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:: Volume 19, Issue 4 (10-2021) ::
Int J Radiat Res 2021, 19(4): 779-789 Back to browse issues page
Liangxue Guyuan decoction alleviates radiation enteritis through PI3K/AKT pathway in rats
Y.G. Wang, H.C. Xu, J. Feng, C.Y. Xu, Y.Q. Dou
Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China , dyqi_301@163.com
Abstract:   (301 Views)
Background: Liangxue Guyuan decoction (LGD) has been used to alleviate symptoms of radiation enteritis (RE) in the clinic. This study aimed to investigate the effects and anti-apoptosis and proliferative mechanisms of LGD in rats with RE. Materials and Methods: A total of 115 male Sprague Dawley rats were used. The control group consisted of 15 rats, and the remaining 100 rats were irradiated with 60Co γ rays at a dose of 11 Gy and randomly divided into four groups of 25 each: the model group, the dexamethasone (DXM) group, the low-dose LGD (LGDl) group, and the high-dose LGD (LGDh) group. The treatment was administered intragastrically for 7 days, and the number of rats that died was recorded daily. On days 3 and 7 after irradiation, five rats were randomly chosen and sacrificed from each group, and ileum samples were taken for pathological observation, immunofluorescence staining, western blotting, and PCR experiments. Results: Compared with the model group, the mean survival time, villus length (V), and the ratio of V to crypt depth C (V/C), the number of Ki67-positive cells was increased, and the number of TUNEL-positive cells was reduced in the LGDh group. In addition, LGDh showed some effects on upregulating p-PI3K, p-AKT, and Bcl-2 and downregulating caspase-3 and Bax. The effect of LGD was better than that of DXM. Conclusions: LGDh prolonged the survival time of irradiated rats, inhibited intestinal epithelial cell apoptosis, and promoted intestinal repair. This mechanism may be related to the regulation of the PI3K/AKT pathway.
Keywords: Liangxue Guyuan decoction, radiation enteritis, PI3K/AKT pathway.
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Type of Study: Original Research | Subject: Radiation Biology
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Wang Y, Xu H, Feng J, Xu C, Dou Y. Liangxue Guyuan decoction alleviates radiation enteritis through PI3K/AKT pathway in rats. Int J Radiat Res. 2021; 19 (4) :779-789
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Volume 19, Issue 4 (10-2021) Back to browse issues page
International Journal of Radiation Research
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