:: Volume 13, Issue 3 (7-2015) ::
Int J Radiat Res 2015, 13(3): 235-241 Back to browse issues page
Development of methemoglobin-based biological dosimetry in gamma–irradiated mice
X.H. Zhang, Y.N. Zhang, X.Y. Min, Z.C. Lou, A.L. Wang, X.D. Hu, H.Q. Zhang
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China , zhanghq@nuaa.edu.cn
Abstract:   (7231 Views)

Background: A new biological dosimeter based on methemoglobin level was developed in this study. Materials and Methods: Methemoglobin level in erythrocytes from mice subjected to &gamma rays from a 60Co source was detected using the methemoglobin kit. The dose range was from 0.5 to 8 Gy and the dose rate was 0.5 Gy/min. Results: The results demonstrate that methemoglobin level increases with increasing dose. The detection limit based on methemoglobin has a lower limit of dose estimation of about 1 Gy. The high levels of methemoglobin are maintained for at least 28 days, and the maximal increase of methemoglobin observed occurs at about 30 min after &gamma irradiation. The relationship between dosage and the increased methemoglobin level can be expressed by a linear quadratic equation of y = -8.75 x2 + 168.09 x + 32.66, with the correlation coefficient, r, equal to 0.96. The best suggested time for blood collection is up to 1 day after &gamma irradiation. The doses absorbed by mice as estimated from the use of the dose-response relationship were close to the blind doses of 1, 2, 4 and 8 Gy. Conclusion: Methemoglobin is a quick, simple, and precise biomarker for the early assessment of the absorbed dose in mice.

Keywords: : Biological dosimetry, methemoglobin, gamma irradiation, mouse
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Type of Study: Original Research | Subject: Radiation Biology

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Volume 13, Issue 3 (7-2015) Back to browse issues page