Effects of different doses of X-ray radiation on nerve regeneration after sciatic nerve injury in a rat model
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Y. Zhang , B. Jiang , J.J. Zhao , J.P. Zhou , Z.C. Zuo , Z.Y. Qian , P.J. Wang |
Department of Hand and Foot Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China , wangpeiji88 |
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Abstract: (2859 Views) |
Background: Physical agents, such as ultrasound, can promote functional restoration and regenerative processes of the peripheral nervous system. However, little is known about the effects of X-ray radiation on nerve regeneration after peripheral nerve injury. The aim of the present study was to investigate the effects of various doses of X-ray radiation on nerve regeneration after sciatic nerve injury in rats. Materials and Methods: The sciatic nerves of Sprague-Dawley rats were transected and repaired via epineurium end-to-end neurorrhaphy. Eighty rats each received single and local X-ray doses of 0 Gy, 0.2 Gy, 1 Gy, 7 Gy and 14 Gy. Functional and morphological assessments of the process of nerve regeneration were performed by using various measurement tools. Results: Compared with the 0 Gy, 0.2 Gy and 14 Gy groups, the 1 Gy and 7 Gy radiation groups experienced significantly increased sciatic functional index, motor nerve conductive velocity (MNCV), expression of S-100, mean diameter of axons, and thickness of myelin sheaths and decreased perineural scar tissue. There were no differences between the 1 Gy group and the 7 Gy group or between the 0 Gy group, the 0.2 Gy group and the 14 Gy group with the exception of MNCV and the expression level of S-100. Conclusion: X-ray radiation in doses of 1 Gy and 7 Gy promoted nerve regeneration after sciatic nerve injury in a rat model. The dose of 14 Gy exerted inhibitory effects, and 0.2 Gy exerted no significant effect on nerve regeneration. |
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Keywords: Peripheral nerve injury, nerve regeneration, X-ray irradiation. |
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Full-Text [PDF 2744 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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