:: Volume 21, Issue 1 (1-2023) ::
Int J Radiat Res 2023, 21(1): 89-95 Back to browse issues page
Radioprotective effect of Nigella sativa Oil (NSO) against radiation-induced hepatic toxicity and haematological alteration in irradiated albino mice
S.S. Mohammed Amin , L. MA. Mohammed , A.B. Faiq , S.S. Salih , Z.Y. Saeed , T.F. Muhammed Salih , D. Abdullah Rashid
Medical Laboratory Department, College of Health & Medical Technology, Sulaimani Polytechnic University, Sulaimaniyeh, Iraq , ary.faiq@spu.edu.iq
Abstract:   (663 Views)
Background: In this study, an attempt was made to estimate to what degree Nigella sativa oil (NSO) could reduce the destruction produced by irradiation on the haematopoietic system, liver function and histopathological changes of liver tissues, and malondialdehyde MDA level in albino mice exposed to whole-body x-radiation. Materials and Methods: the mice were divided into four different groups. Group I (negative control group) received deionized water. Group II (6 Gy group) was exposed to whole-body X-irradiation at 6 Gy. Group III (NSO) received NSO orally (10 ml/kg). Group IV (NSO+6 Gy) received NSO orally (10 ml/kg) for 5 days prior to irradiation. Results: The results of the group exposed to 6 Gy X-irradiation showed a decrease in red blood cell count, haemoglobin, hematocrit, mean corpuscular volume and mean corpuscular haemoglobin (all with P ≤ 0.05), while for the group that received NSO before irradiation, all the mentioned parameters were ameliorated and shifted toward normal values. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme levels were showed to be affected: they both increased during irradiation in the (6 Gy) group and decreased in the (NSO + 6 Gy) group. Histopathological alterations were also observed when the liver was irradiated in the (6 Gy) group. However, the (NSO+6 Gy) group demonstrated a lowering of the incidence of histopathological changes in liver tissues. Conclusion: it is clear that mice treated with NSO oil prior to X-irradiation had reduced alteration in the haematological parameters, hepatocyte lipid peroxidation and the histology of the liver.
Keywords: X- radiation, nigella sativa oil (NSO), haematological parameters, lipid peroxidation, histological modification, liver function.
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Type of Study: Original Research | Subject: Radiation Biology
References
1. Desouky O, Ding N, Zhoub, G (2015) Targeted and non-targeted effects of ionizing radiation. J Radiat Res Appl Sci, 8(2): 247-54. [DOI:10.1016/j.jrras.2015.03.003]
2. Desouky O, Ding N, Zhou G (1970) Direct and indirect action of radiation. In Molecular Radiation Biology (pp. 70-90). New York, NY: Springer. [DOI:10.1007/978-1-4684-6247-0_6]
3. Painuli S and Kumar N (2016) Prospects in the development of natural radioprotective therapeutics with anti-cancer properties from the plants of Uttarakhand region of India. J Ayurveda Integr Med, 7(1): 62-68. [DOI:10.1016/j.jaim.2015.09.001] [PMID] []
4. Tan BL, Norhaizan ME, Huynh K, Heshu SR, Yeap, SK, Hazilawati, H, Roselina, K (2015) Water extract of brewers' rice induces apoptosis in human colorectal cancer cells via activation of caspase-3 and caspase-8 and downregulates the Wnt/β-catenin downstream signaling pathway in brewers' rice-treated rats with azoxymethane-induced colon carc. BMC CAM, 15(1): 205. [DOI:10.1186/s12906-015-0730-4] [PMID] []
5. Alenzi FQ, Altamimi MA, Kujan O, Tarakji B, Tamimi W, Bagader O, . . . Wys RK (2013) Antioxidant properties of Nigella sativa. J Mol Genet Med, 7(3): 1-5. [DOI:10.4172/1747-0862.1000077]
6. Mollazadeh H and Hosseinzadeh H (2014) The protective effect of Nigella sativa against liver injury. Iran J Basic Med Sci, 17(12): 958-66.
7. Velho-Pereira R, Kumar A, Pandey BN, Mishra KP, Jagtap AG (2012) Radioprotection by macerated extract of Nigella sativa in normal tissues of fibrosarcoma bearing mice. Indian J Pharm Sci, 74(5): 403-14. [DOI:10.4103/0250-474X.108415] [PMID] []
8. Williams JP, Brown SL, Georges GE, Hauer-Jensen M, Hill RP, Huser AK, et al. (2010) Animal models for medical countermeasures to radiation exposure. Radiat Res, 173(4): 557-78. [DOI:10.1667/RR1880.1] [PMID] []
9. Tan BL, Norhaizan ME, Liew WPP, Rahman HS (2018) Antioxidant and oxidative stress: A mutual interplay in age-related diseases. Front Pharmacol, 9: 1162. [DOI:10.3389/fphar.2018.01162] [PMID] []
10. Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res, 14(5): 323-28. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q [DOI:10.1002/1099-1573(200008)14:53.0.CO;2-Q]
11. Bordoni L, Fedeli D, Nasuti C, Maggi F, Papa F, Wabitsch M, et al. (2019) Antioxidant and anti-Inflammatory properties of nigella sativa oil in human pre-adipocytes. Antioxidants, 8(2): 51. [DOI:10.3390/antiox8020051] [PMID] []
12. Khan MA, Chen HC, Tania M, Zhang DZ (2011) Anticancer activities of nigella sativa (black cumin). Afr J Tradit Complement Altern Med, 8(5): 226-32. [DOI:10.4314/ajtcam.v8i5S.10] [PMID] []
13. Bourgou S, Ksouri R, Bellila A, Skandrani I, Falleh H, Marzouk B (2008). Phenolic composition and biological activities of Tunisian Nigella sativa L. shoots and roots. C R biol, 331(1): 48-55. [DOI:10.1016/j.crvi.2007.11.001] [PMID]
14. Anter M, Coskun O, Budancamanak M (2005) Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol, 11(42): 6684-88. [DOI:10.3748/wjg.v11.i42.6684] [PMID] []
15. Meral I, Yener I, Kahraman I, Mert N (2001) Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti‐oxidant defence system and liver damage in experimentally‐Induced diabetic rabbits. J Vet Med A Physiol Pathol Clin Med, 48(10): 593-99. [DOI:10.1046/j.1439-0442.2001.00393.x] [PMID]
16. Abdel-Hamid NM, Abdel-Ghany MI, Nazmy MH, Amhad SW (2013) Can methanolic extract of Nigella sativa seed affect glyco-regulatory enzymes in experimental hepatocellular carcinoma? Environ Health Prev Med, 18: 49-56. [DOI:10.1007/s12199-012-0292-8] [PMID] []
17. Farah IO and Begum RA (2003) Effect of Nigella sativa (N. sativa L.) and oxidative stress on the survival pattern of MCF-7 breast cancer cells. Biomed Sci Instrum, 39: 359-64.
18. Sun Q, Mao W, Jiang H, Zhang X, Xiao J, Lian Y (2018) The Effect of protracted exposure to radiation on liver injury: A cohort study of industrial. Int J Environ Res Public Health, 15(1): 71. [DOI:10.3390/ijerph15010071] [PMID] []
19. Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB (2000) Diagnosis and monitoring of hepatic injury. I. performance characteristics of laboratory tests. Clin Chem, 46(12): 2027-2049. [DOI:10.1093/clinchem/46.12.2027] [PMID]
20. Xu D, Peng M, Zhang Z, Dong G, Zhang Y, Yu H (2012) Study of damage to red blood cells exposed to different doses of γ-ray irradiation. Blood Transfus, 10(3): 321-30.
21. Sancheti G and Goyal PK (2007) Prevention of radiation induced haematological alterations by medicinal plant Rosmarinus officinalis, in mice. Afr J Tradit Complement Altern Med, 4(2): 165-72. [DOI:10.4314/ajtcam.v4i2.31205] [PMID] []
22. Agarwal M, Purohit RK, Chakrawarti A, Basu A, Bhartyia KM (2011) Protective efficacy of Aloe vera against radiation and cadmium induced haematological changes in the Swiss albino mice. Advanced Biotech, 10(10): 44-70.
23. Sharma R, Purohit RK, Sharma S, Rao R (2012) Protective role of liv. 52 against radiation and Cadmium induced haematological changes in the Swiss albino mice. Proceedings of the international conference on emerging frontiers and challenges in radiation biology, India.
24. El-Deeb AE, Abd-Aleem IM, Abd-Rahman AA (2006) The curative effect of some antioxidants on γ-irradiated rats. Egypt Soc Toxicol, 36: 79-89.
25. Mehrotra S, Pecaut MJ, Gridley DS (2013) Effects of minocycline on haematopoietic recovery after whole-body irradiation. In Vivo, 27(1): 11-28.
26. Assayed ME (2010) Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Immunopharmacol Immunotoxicol, 32(2): 284-96. [DOI:10.3109/08923970903307552] [PMID]
27. Najam LA, AL-Dulamey QK, Al-Jawwady YA (2020) Effects of low dose Gamma ray on some haematological parameters in adult rats. Iran. J. Medical Phys, 17: 137-41.
28. JP T (2014) Black seed oil cures many cancer according to numerous studies. Health Impact News, 16(11): 1-15.
29. Fabiani R, Rosignoli P, Bartolomeo AD, Fuccelli R, Servili M, Montedoro GF, Morozzi G (2008) Oxidative DNA damage is prevented by extracts of olive oil, hydroxytyrosol, and other olive phenolic coumpounds in human blood mononuclear cells and HL60 cells. Nutr, 138(8): 1411-16. [DOI:10.1093/jn/138.8.1411] [PMID]
30. Nakajima T, Ninomiya Y, Nenoi M (2019) Radiation-induced reactions in the liver modulation of radiation effects by lifestyle-related factors. Int J Mol Sci, 19(12): 3855. [DOI:10.3390/ijms19123855] [PMID] []
31. Benson R, Madan R, Kilambi R, Chander S (2016) Radiation induced liver disease. J Egypt Natl Canc Inst, 28(1): 7-11. [DOI:10.1016/j.jnci.2015.08.001] [PMID]
32. Ahmad A and Alkreathy HM (2018) Comparative biochemical and histopathological studies on the efficacy of metformin and Nigella sativa oil against thioacetamide-induced acute hepatorenal damage in rats. Biomed Res, 29(15): 3106-16. [DOI:10.4066/biomedicalresearch.29-18-914]
33. Cheikh-Rouhoua S, Besbes S, Hentati B, Blecker C, Deroanne C, Attiaa H (2007) Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction. Food Chem, 101(2): 673-81. [DOI:10.1016/j.foodchem.2006.02.022]
34. Dollah MA, Parhizkar S, Latiff LA, Hassan MH (2013) Toxicity effect of Nigella sativa on the liver function of rats. Adv Pharm Bull, 3(1): 97-102.
35. Essawy AE, Abdel-Moneim AM, Khayyat LI, Elzergy AA (2012) Nigella sativa seeds protect against hepatotoxicity and dyslipidemia induced by carbon tetrachloride in mice. J App Pharm Sci, 2(10): 21-25. [DOI:10.7324/JAPS.2012.21004]
36. Reed GB and Cox AJJr (1966) The human liver after radiation injury. A form of veno-occlusive disease. Am J Pathol, 48(4): 597-611.
37. Takamatsu S, Kozaka K, Kobayashi S, Yoneda N, Yoshida K, Inoue D, et al. (2018) Pathology and images of radiation-induced hepatitis: a review article. Jpn J Radiol, 36(4): 241-56. [DOI:10.1007/s11604-018-0728-1] [PMID]



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