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:: Volume 21, Issue 2 (4-2023) ::
Int J Radiat Res 2023, 21(2): 325-330 Back to browse issues page
Dysregulation of miR-711 exerts a protective role against the X-ray-induced-injury in human umbilical vein endothelial cells
Z. Liu, Y. Wang, H. Xin, Sh. Sun
Vascular Interventional Surgery Department, Affiliated Hospital of Weifang Medical University, Shandong, 261041, China , shun8300duixu@163.com
Abstract:   (72 Views)
Background: Radiation therapy inevitably causes certain radiation damage to normal cells such as human vascular endothelial cells (HVECs), in addition to eliminating tumor cells. This article discusses the influence of miR-711 on the survival and apoptosis of human umbilical vein endothelial cells (HUVECs) after X-ray radiation. Materials and Methods: The expression of miR-711 and v-maf musculoaponeurotic fibrosarcoma oncogene family protein G (MAFG) was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and the content of vascular cell adhesion molecule 1 (VCAM-1) in serum was detected by Enzyme-linked immunosorbent assay (ELISA). The survival and apoptosis of HUVECs after X-ray irradiation were analyzed by functional experiments, and validation of MAFG as a miR-711 target was done by dual-luciferase reporter assay. Results: In the serum of prostate cancer patients miR-711 was up-regulated, while MAFG was down-regulated compared to the control group. After X-ray radiation intervention, miR-711 levels were increased, in contrast, MAFG levels decreased with a concomitant increase in VCAM-1 expression.. The percentage of survival level of HUVECs decreased gradually and the apoptosis rate of HUVECs increased as the dosage of X-ray radiation increased. Notably, after 2Gy X-ray radiation, the miR-711 inhibitor increased the survival rate of HUVECs while reducing the apoptosis rate, in contrast to the miR-711 mimic decreased the survival rate of HUVECs and increased the apoptosis. Besides, luciferase activity assay demonstrated the targeting of miR-711 to MAFG. Conclusion: The inhibition of miR-711 might abrogate the function of X-ray irradiation on the survival and apoptosis of HUVECs.
Keywords: miR-711, MAFG, HUVECs, X-ray, percentage of survival, apoptosis.
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Type of Study: Original Research | Subject: Radiation Biology
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Liu Z, Wang Y, Xin H, Sun S. Dysregulation of miR-711 exerts a protective role against the X-ray-induced-injury in human umbilical vein endothelial cells. Int J Radiat Res 2023; 21 (2) :325-330
URL: http://ijrr.com/article-1-4771-en.html

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Volume 21, Issue 2 (4-2023) Back to browse issues page
International Journal of Radiation Research
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