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Dysregulation of miR-711 exerts a protective role against the X-ray-induced-injury in human umbilical vein endothelial cells
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| Z. Liu, Y. Wang, H. Xin, Sh. Sun |
| Vascular Interventional Surgery Department, Affiliated Hospital of Weifang Medical University, Shandong, 261041, China , shun8300duixu@163.com |
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Abstract: (72 Views) |
Background: Radiation therapy inevitably causes certain radiation damage to normal cells such as human vascular endothelial cells (HVECs), in addition to eliminating tumor cells. This article discusses the influence of miR-711 on the survival and apoptosis of human umbilical vein endothelial cells (HUVECs) after X-ray radiation. Materials and Methods: The expression of miR-711 and v-maf musculoaponeurotic fibrosarcoma oncogene family protein G (MAFG) was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and the content of vascular cell adhesion molecule 1 (VCAM-1) in serum was detected by Enzyme-linked immunosorbent assay (ELISA). The survival and apoptosis of HUVECs after X-ray irradiation were analyzed by functional experiments, and validation of MAFG as a miR-711 target was done by dual-luciferase reporter assay. Results: In the serum of prostate cancer patients miR-711 was up-regulated, while MAFG was down-regulated compared to the control group. After X-ray radiation intervention, miR-711 levels were increased, in contrast, MAFG levels decreased with a concomitant increase in VCAM-1 expression.. The percentage of survival level of HUVECs decreased gradually and the apoptosis rate of HUVECs increased as the dosage of X-ray radiation increased. Notably, after 2Gy X-ray radiation, the miR-711 inhibitor increased the survival rate of HUVECs while reducing the apoptosis rate, in contrast to the miR-711 mimic decreased the survival rate of HUVECs and increased the apoptosis. Besides, luciferase activity assay demonstrated the targeting of miR-711 to MAFG. Conclusion: The inhibition of miR-711 might abrogate the function of X-ray irradiation on the survival and apoptosis of HUVECs. |
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| Keywords: miR-711, MAFG, HUVECs, X-ray, percentage of survival, apoptosis. |
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Full-Text [PDF 679 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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