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Induction of chromosomal aberrations in human primary fibroblasts and immortalized cancer cells exposed to extremely-low-frequency electromagnetic fields
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S.S. Seyyedi   , H. Mozdarani , M. Rezaei Tavirani , S. Heydari |
| , mozdarah@modares.ac.ir |
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Abstract: (13840 Views) |
| Background: Rapidly increasing possibilities of
exposure to environmental extremely low-frequency
electromagnetic fields (ELF-EMF) have become a
topic of worldwide investigation. Epidemiological and
laboratory studies suggest that exposure to ELF-EMF
may increase cancer risk therefore assessment of
chromosomal damage in various cell lines might be of
predictive value for future risk estimation. Materials
and Methods: Primary cultures of fibroblasts from
human skin biopsy were exposed to continuous
extremely low-frequency electromagnetic fields (3, 50
and 60 Hz, sinusoidal, 3h, and 4 mT). Also immortalized
cell lines, SW480, MCF-7 and 1321N1 were
exposed to continuous ELF-EMF (50 Hz, sinusoidal, 3
h, 4 mT). Metaphase plates were prepared according
to standard methods and stained in 5% Giemsa
solution. Chromosomal aberrations of both chromosome
and chromatid types were scored to evaluate
the effects of ELF-EMF on primary or established cell
lines. Results: Results indicate that by increasing the
frequency of ELF-EMF, chromosomal aberrations
were increased up to 7-fold above background levels
in primary human fibroblast cells. In addition, continuous
exposure to a 50 Hz electromagnetic field led to
a significant increase in chromosomal aberrations in
SW480, MCF-7 and 1321N1 cell lines compared to
sham control. Conclusion: Results obtained indicate
that ELF-EMF has the potential for induction of
chromosomal aberrations in all cell types. Iran. J.
Radiat. Res., 2010 8 (1): 25-29 |
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| Keywords: ELF-EMF, chromosomal aberrations, continuous exposure, human fibroblasts, cancer cell lines. |
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Full-Text [PDF 243 kb]
(2602 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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