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Dose estimation with the calibration of dose-response curve of micronucleus in human peripheral lymphocytes induced by 50MeV proton beams
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Y.J. Go   , J.H. Shin , K.S. Jeong , S.J. Park , S.H. Kim , S.Y. Ryu , C.H. Kim , E.J. Kim , Ch.M. Kang , TH. Kim |
| , thkim56@knu.ac.kr |
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Abstract: (13047 Views) |
| Background: The purpose of this paper is to
establish an easy and reliable biodosimeter protocol
to evaluate the biological effects of proton beams.
Materials and Methods: Human peripheral blood
lymphocytes were irradiated using proton beams (LET:
34.6 keV μm−1), and the chromosome aberrations
induced were analyzed using cytokinesis-blocked (CB)
micronucleus (MN) assay. To determine the efficiency
of MN assay in estimating the doses received by
50MeV proton beams and to monitor predicted dose
of victims in accidental exposure, here we have
evaluated the performance of MN analysis in a
simulated situation after exposure with proton
beams. Peripheral lymphocytes were irradiated by
50MeV proton beams up to 6Gy and analyzed by
Giemsa staining of CB MN assay. Results: The
detected MN was found to be a significant dose-effect
curve in the manner of dose-dependent increase
after exposure with proton beams in vitro. When
plotting on a linear scale against radiation dose, the
line of best fit was Y=0.004+(1.882x10-2±9.701x10-5) D+(1.43x10-3±1.571x10-5)D2. Our results show a
trend towards increase of the number of MN with
increasing dose. It was linear-quadratic and has a
significant relationship between the frequencies of
MN and dose (R2= 0.9996). The number of MN in
lymphocyte that was observed in control group is
5.202±0.04/cell. Conclusion: Hence, this simple
protocol will be particularly useful for helping physicians
to decide medical therapy for the initial treatment
of victims with rapid and precise dose estimation
after accidental radiation exposure. Also it has
potential for use as a valuable biomarker to evaluate
the biological effectiveness for cancer therapy with
proton beams. Iran. J. Radiat. Res., 2011 8(4): 231-236 |
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| Keywords: Human peripheral lymphocyte, biodosimetry, proton beams, dose estimation, triage. |
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Full-Text [PDF 353 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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