AU - Mozdarani, H. AU - Taheri, A. AU - Haeri, S.A. TI - Assessment of the radioprotective effects of amifostine on human lymphocytes irradiated in vitro by gamma-rays using cytokinesis-blocked micronucleus assay PT - JOURNAL ARTICLE TA - Int-J-Radiat-Res JN - Int-J-Radiat-Res VO - 5 VI - 1 IP - 1 4099 - http://ijrr.com/article-1-288-en.html 4100 - http://ijrr.com/article-1-288-en.pdf SO - Int-J-Radiat-Res 1 AB  -   Background: A radioprotective effect of amifostine as well as its ability to modulate the level of spontaneous and gamma-irradiation-induced genetic changes on human peripheral blood lymphocytes has been investigated . Amifostine, known as a potent radical scavenger, has been introduced as the most effective radioprotector, yet it is not completely approved for the clinical use. However, further in vitro and clinical studies are needed to clarify its mechanisms of action.   Materials and Methods : Whole blood samples from healthy donors were exposed to various doses of gamma-rays. Lymphocytes in cultures were treated with amifostine at different concentrations (2, 4 and 6 mM) in the presence or in the absence of 1 IU/ml alkaline phosphatase before or after gamma-irradiation. Standard procedure for the cytokinesis-block micronucleus (CBMN) assay was used to assess the effect of amifostine on radiation induced micronucleus in binucleate lymphocytes.     Results : Irradiated blood samples showed an increase in the total number of micronuclei (MN) significantly different from controls (p CP - IRAN IN - 1Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran LG - eng PB - Int-J-Radiat-Res PG - 9 PT - Original Research YR - 2007