International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1394
4
1
gregorian
2015
7
1
13
3
online
1
fulltext
en
Development of methemoglobin-based biological dosimetry in gamma–irradiated mice
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<p><strong>Background:</strong> A new biological dosimeter based on methemoglobin level was developed in this study. <strong>Materials and Methods:</strong> Methemoglobin level in erythrocytes from mice subjected to &gamma rays from a <sup>60</sup>Co source was detected using the methemoglobin kit. The dose range was from 0.5 to 8 Gy and the dose rate was 0.5 Gy/min. <strong>Results:</strong> The results demonstrate that methemoglobin level increases with increasing dose. The detection limit based on methemoglobin has a lower limit of dose estimation of about 1 Gy. The high levels of methemoglobin are maintained for at least 28 days, and the maximal increase of methemoglobin observed occurs at about 30 min after &gamma irradiation. The relationship between dosage and the increased methemoglobin level can be expressed by a linear quadratic equation of y = -8.75 x2 + 168.09 x + 32.66, with the correlation coefficient, r, equal to 0.96. The best suggested time for blood collection is up to 1 day after &gamma irradiation. The doses absorbed by mice as estimated from the use of the dose-response relationship were close to the blind doses of 1, 2, 4 and 8 Gy. <strong>Conclusion:</strong> Methemoglobin is a quick, simple, and precise biomarker for the early assessment of the absorbed dose in mice.</p>
: Biological dosimetry, methemoglobin, gamma irradiation, mouse
235
241
http://ijrr.com/browse.php?a_code=A-10-1-567&slc_lang=en&sid=1
X.H.
Zhang
790031947532846009819
790031947532846009819
No
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China
Y.N.
Zhang
790031947532846009820
790031947532846009820
No
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China
X.Y.
Min
790031947532846009821
790031947532846009821
No
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China
Z.C.
Lou
790031947532846009822
790031947532846009822
No
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China
A.L.
Wang
790031947532846009823
790031947532846009823
No
The First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu, Anhui, P.R. China
X.D.
Hu
790031947532846009824
790031947532846009824
No
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China
H.Q.
Zhang
zhanghq@nuaa.edu.cn
790031947532846009825
790031947532846009825
Yes
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China