en Radiation Biology Radiation Biology تحقيق بديع Original Research <span style="font-weight: bold"> Background:</span> Radiolabeled polyclonal human immunoglubins are useful in the detection of inflammations. In this work a novel approach has been presented to use thallium-201 as a comlexed nuclide for the development of radioimmunoconjugates. <span style="font-weight: bold">Materials and Methods:</span> Thallium-201 (T1/2=3.04 d) in Tl+ form was converted to Tl3+ cation in presence of O3 in 6M HCl, controlled by RTLC/gel electrophoresis methods and used in the labeling of human polyclonal antibody (HIgG) after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a HIgG pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7) to a glass tube, which was pre-coated with DTPA-dianhydride (0.01 mg) at 25C with continuous mild stirring for 30 min. <span style="font-weight: bold">Results:</span> The final isotonic [201Tl](III)-DTPA-HIgG complex was checked by radio-TLC using several solvent systems to ensure the formation of only one species, and it was followed by filtration through a 0.22 µm filter (specific activity= 33.7 TBq/mM, radiochemical purity95%). Preliminary bio-distribution studies in normal and inflammation-bearing rats were performed. The target/skin and target/blood ratios were 4 and 6 after 28h, respectively, showing the selectivity of the radiopharmaceutical for the inflammatory lesions. <span style="font-weight: bold">Conclusion:</span> The incorporation of Tl(III) cation into a immunoconjugate was performed using the known methods. The biodistribution of the immunocomplex was shown to be consistent with a stable complex for the detection of inflammations. Significant inflammation detection was observed for the final complex in rats with turpentine oil-induced inflammation. 201Tl(III)-complex, biodistribution, human polyclonal antibody, inflammation 105 114 http://ijrr.com/browse.php?a_code=A-10-1-209&slc_lang=en&sid=1 A. R. Jalilian ajalilian@nrcam.org 79003194753284600635 79003194753284600635 No A. Khorrami 79003194753284600636 79003194753284600636 No M. B. Tavakoli 79003194753284600637 79003194753284600637 No M. Kamali-dehghan 79003194753284600638 79003194753284600638 No Y. . Yari Kamrani 79003194753284600639 79003194753284600639 No F. Shahidi 79003194753284600640 79003194753284600640 No