International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1398
10
1
gregorian
2020
1
1
18
1
online
1
fulltext
en
Prophylactic effect of Spirulina platensis on radiation-induced thyroid disorders and alteration of reproductive hormones in female albino rats
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
ABSTRACT
<div style="text-align: justify;">Background: Ionizing-radiation induces oxidative stress and thyroid toxicity. Thyroid function disorders have a great impact on fertility in both sexes. Materials and Methods: Forty female rats were divided into four groups. Control, Spirulina-treated (300 mg/kg); given orally for 15 days, γ-irradiated; given (5 Gy whole body γ-rays) and Spirulina+irradiated; given Spirulina for 15 days before irradiation. Animals were sacrificed the 3<sup>rd</sup> day post-irradiation. The level of the oxidant/antioxidant markers: Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) was evaluated. In addition, caspase-3 activity was measured as apoptotic marker and comet assay to detect DNA-damage. Serum thyroid stimulating hormone (TSH), triiodothyronine (T3) and thyroxine (T4) were determined to evaluate the thyroid function alterations. Also, analysis of reproductive hormones; follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P4) was detected. Results: Whole body γ-irradiation-induced oxidative stress, denoted by significant decreases of antioxidant markers and an increase in MDA content. The activity of caspase-3 was significantly increased and comet assay revealed DNA damage. Also, serum level of TSH was significantly increased, while T3, and T4, significantly decreased in irradiated rats. Moreover, the reproductive hormones showed significant decreases. Spirulina treatment has significantly attenuated oxidative stress in thyroid tissues, decreased caspase-3 activity and ameliorated DNA damage, concomitant with significant amelioration in the levels of thyroid and reproductive hormones. Conclusion: Spirulina may alleviate γ-rays-induced thyroid damage and play a significant role in the regulation of thyroid and reproductive hormones in female rats. </div>
Spirulina platensis, thyroid, gamma radiation, caspase-3, comet assay.
83
90
http://ijrr.com/browse.php?a_code=A-10-1-842&slc_lang=en&sid=1
R.M.
Ebrahim
Rehab.2000@hotmail.com
7900319475328460015402
7900319475328460015402
Yes
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Nasr City, Cairo, Egypt