en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: To investigate effect of radiosensitization of [Ru(bpy)₂(phen)]Cl₂ complex on nasopharyngeal carcinoma cell line CNE1 and its mechanism. Materials and Methods: Nasopharyngeal carcinoma cell line CNE1 in vitro culture was divided into control group, light irradiation group (4 Gy, 6 MV photonic line), simple metal ruthenium complex treatment group (Ru group, 100 &mu;mol/L [Ru(bpy)₂(phen)]Cl₂) and metal ruthenium complex combined with radiotherapy group (Combined radiotherapy group, cells were irradiated with 4 Gy and 6 MV photons at 2 days after 100 &mu;mol/L [Ru(bpy)₂(phen)]Cl₂). Results: Transcriptional level of P53 gene in combined radiotherapy group was higher than that in the other groups (P<0.001). Inhibition rate of combined radiotherapy group was higher than that of Ru group and irradiation group (P<0.001). Apoptotic rate was the highest (P<0.05) in the combined radiotherapy group, and irradiation group was higher than Ru group and control group (P<0.05). Survival rate of Ru group was lower than that of control group under the same radiation dose (P<0.05), and the radiotherapy sensitization ratio was 1.227 (Dq ratio). Conclusion: [Ru(bpy)₂(phen)]Cl₂ increases the sensitivity of nasopharyngeal carcinoma cell line CNE1 to X-ray, which may be related to increase of P53 gene expression.</div> [Ru(bpy)2(phen)]Cl2, nasopharyngeal carcinoma cells, P53 gene, radiosensitization. 549 555 http://ijrr.com/browse.php?a_code=A-10-1-862&slc_lang=en&sid=1 J. Chen jw_chen18@outlook.com 7900319475328460017309 7900319475328460017309 Yes Department of Radiotherapy, Fujian Medical University Union Hospital, Fuzhou 350001, and the Third Affiliated Hospital, Fujian Medical University, Fuzhou 350108, People's Republic of China Y. Xiao 7900319475328460017310 7900319475328460017310 No State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou 350002, People's Republic of China L. Wu 7900319475328460017311 7900319475328460017311 No The Second Clinical Medical College, Fujian Medical University, Quanzhou 362000, People's Republic of China F. Xiao 7900319475328460017312 7900319475328460017312 No State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou 350002, People's Republic of China Q. Wang 7900319475328460017313 7900319475328460017313 No Department of Oncology, The First people’s Hospital of Jingde Municipal, Jingdezhen 333000, People's Republic of China J. Hong 7900319475328460017314 7900319475328460017314 No Department of Radiotherapy, the First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, People's Republic of China P. Zhu 7900319475328460017315 7900319475328460017315 No Department of Pathogenic Biology, Basic Medicine College, Fujian Medical University, Fuzhou 350122, People's Republic of China