en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Liangxue Guyuan decoction (LGD) has been used to alleviate symptoms of radiation enteritis (RE) in the clinic. This study aimed to investigate the effects and anti-apoptosis and proliferative mechanisms of LGD in rats with RE. Materials and Methods: A total of 115 male Sprague Dawley rats were used. The control group consisted of 15 rats, and the remaining 100 rats were irradiated with ⁶⁰Co &gamma; rays at a dose of 11 Gy and randomly divided into four groups of 25 each: the model group, the dexamethasone (DXM) group, the low-dose LGD (LGDl) group, and the high-dose LGD (LGDh) group. The treatment was administered intragastrically for 7 days, and the number of rats that died was recorded daily. On days 3 and 7 after irradiation, five rats were randomly chosen and sacrificed from each group, and ileum samples were taken for pathological observation, immunofluorescence staining, western blotting, and PCR experiments. Results: Compared with the model group, the mean survival time, villus length (V), and the ratio of V to crypt depth C (V/C), the number of Ki67-positive cells was increased, and the number of TUNEL-positive cells was reduced in the LGDh group. In addition, LGDh showed some effects on upregulating p-PI3K, p-AKT, and Bcl-2 and downregulating caspase-3 and Bax. The effect of LGD was better than that of DXM. Conclusions: LGDh prolonged the survival time of irradiated rats, inhibited intestinal epithelial cell apoptosis, and promoted intestinal repair. This mechanism may be related to the regulation of the PI3K/AKT pathway.</div> Liangxue Guyuan decoction, radiation enteritis, PI3K/AKT pathway. 779 789 http://ijrr.com/browse.php?a_code=A-10-1-867&slc_lang=en&sid=1 Y.G. Wang 7900319475328460020373 7900319475328460020373 No Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China H.C. Xu 7900319475328460020374 7900319475328460020374 No Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China J. Feng 7900319475328460020375 7900319475328460020375 No Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China C.Y. Xu 7900319475328460020376 7900319475328460020376 No Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China Y.Q. Dou dyqi_301@163.com 7900319475328460020377 7900319475328460020377 Yes Senior Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China